Optical Nano-mapping and Analysis of Plant Genomes

Methods in Molecular Biology
Ming-Cheng LuoMichael Saghbini


Application of optical mapping based on BioNano Genomics Irys(®) technology ( http://www.bionanogenomics.com/ ) is growing rapidly since its debut in November 2012. The technology can be used to facilitate genome sequence assembly and analysis of genome structural variations. We describe here the detailed protocol that we used to generate a whole genome BioNano map for Aegilops tauschii, the D genome progenitor of hexaploid wheat (Triticum aestivum). We are using the whole genome BioNano map to validate sequence assembly based on the next-generation sequencing, order sequence scaffolds, and ultimately build pseudomolecules for the genome.


Jun 9, 2018·Genome Génome / Conseil National De Recherches Canada·Tingting ZhuMing-Cheng Luo
Aug 1, 2018·Nature Genetics·Nathan M SpringerThomas P Brutnell

Related Concepts

Genome Mapping
Aegilops tauschii
Massively-Parallel Sequencing
Triticum aestivum antigen
Sequence Determinations, DNA
Optical Restriction Mapping
High-Throughput Nucleotide Sequencing

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