PMID: 376313Apr 1, 1979Paper

Optical probe responses on sarcoplasmic reticulum: oxacarbocyanines as probes of membrane potential

European Journal of Biochemistry
T BeelerA Martonosi

Abstract

The relationship between Ca2+ fluxes and the ion diffusion potential was analyzed on sarcoplasmic reticulum membranes using oxacarbocyanine dyes as optical probes for membrane potential. 3.3'-Diethyloxodicarbocyanine responds to ATP-induced Ca2+ uptake by isolated sarcoplasmic reticulum vesicles with a decrease in absorbance at 600 nm. The optical change is reversed during Ca2+ release from sarcoplasmic reticulum induced by KCl or by ADP and inorganic phosphate. The absorbance changes are largely attributable to the binding of accumulated Ca2+ to the membrane. There is no indication that sustained changes in membrane diffusion potential would accompany pump-mediated Ca2+ fluxes. A large change in the absorbance of 3,3'-diethyloxodicarbocyanine was observed on sarcoplasmic reticulum vesicles under the influence of membrane potential generated by valinomycin in the presence of a K+ gradient or by ionophore A23187 in the presence of a Ca2+ gradient. The maximum of the potential-dependent absorbance change is at 575--580 nm. The potentials generated by valinomycin or ionophore A23187 are short-lived due to the high permeability of sarcoplasmic reticulum membranes for cations and anions. There is no correlation between the direction...Continue Reading

References

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Dec 30, 1958·The Journal of Physiology·A F HUXLEY, R E TAYLOR

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Citations

Oct 31, 1980·The Journal of Membrane Biology·V C Chiu, D H Haynes
Jan 1, 1981·The Journal of Membrane Biology·T J BeelerA N Martonosi
Jan 30, 1981·The Journal of Membrane Biology·A H Caswell, N R Brandt
Jan 1, 1985·Progress in Biophysics and Molecular Biology·J CailléO Rougier
Mar 1, 1983·The Journal of Cell Biology·S M WolniakW T Jackson
Sep 22, 2006·Doklady. Biochemistry and Biophysics·L R ArtinianL M Chailakhyan
Jan 1, 1982·The Journal of Membrane Biology·J N WeinsteinR D Klausner
Jan 1, 1983·The Journal of Membrane Biology·T Beeler
Feb 1, 1980·Journal of Bacteriology·J Van Brunt, F M Harold

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