Optical probes for molecular processes in live cells

Annual Review of Analytical Chemistry
Yoshio Umezawa

Abstract

In this review, I summarize the development over the past several years of fluorescent and/or bioluminescent indicators to pinpoint cellular processes in living cells. These processes involve second messengers, protein phosphorylations, protein-protein interactions, protein-ligand interactions, nuclear receptor-coregulator interactions, nucleocytoplasmic trafficking of functional proteins, and protein localization.

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·E R FearonC Van Dang
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C T ChienS Fields
Feb 1, 1991·Molecular and Cellular Biology·C V DangE R Fearon
Mar 10, 1995·The Journal of Biological Chemistry·A J CaplanJ Vidal
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·N Johnsson, A Varshavsky
Jun 20, 1997·The Journal of Biological Chemistry·S Chong, M Q Xu
Jun 6, 1998·Nucleic Acids Research·X XuY Luo
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·I StagljarS te Heesen
Jun 10, 1998·Nature·S P Williams, P B Sigler
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·A FloresM Werner
Jan 6, 2000·Nature Cell Biology·M ZaccoloT Pozzan
Feb 12, 2000·Angewandte Chemie·C J NorenF B Perler
May 9, 2000·Nature·L A Cary, J A Cooper
Jun 14, 2000·Current Opinion in Neurobiology·D A ZachariasR Y Tsien
Aug 31, 2000·Annual Review of Biochemistry·H Paulus
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·K Simons, D Toomre
Mar 5, 2002·Nature Biotechnology·Moritoshi SatoYoshio Umezawa
Dec 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bharathi LaxmanAlnawaz Rehemtulla
Jan 4, 2003·Nature Reviews. Molecular Cell Biology·Ralf Lösel, Martin Wehling

❮ Previous
Next ❯

Citations

Oct 12, 2010·Chembiochem : a European Journal of Chemical Biology·Misha V GolynskiyMaarten Merkx
Feb 11, 2014·Analytical Chemistry·Christina M Tyrakowski, Preston T Snee

❮ Previous
Next ❯

Methods Mentioned

BETA
FRET
fluorescence resonance energy
fluorescence imaging
fluorescence
fluorescence resonance
two-hybrid
two-hybrids
dissection
contraception
nuclear translocation

Software Mentioned

Srcus
TM
Simbi

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.