PMID: 6981683Aug 1, 1982Paper

Optical signals from surface and T system membranes in skeletal muscle fibers. Experiments with the potentiometric dye NK2367

The Journal of General Physiology
J A Heiny, J Vergara

Abstract

Absorbance signals were recorded from cut single skeletal muscle fibers stained with the nonpenetrating potentiometric dye NK2367 and mounted in a three-vaseline-gap voltage clamp. The characteristics of the optical signals recorded under current and voltage-clamp conditions were studied at various wavelengths between 500 and 800 nm using unpolarized light. Our results indicate that the absorbance signals recorded with this dye reflect potential changes across both the surface and T system membranes and that the relative contribution of each of these membrane compartments to the total optical change is strongly wavelength dependent. A peak intensity change was detected at 720 nm for the surface membrane signal and at 670 nm for the T system. Evidence for this wavelength-dependent separation derives from an analysis of the kinetics and voltage dependence of the optical signals at different wavelengths, and results obtained in detubulated fibers. The 670-nm optical signal was used to demonstrate the lack of potential control in the T system by the voltage clamp and the effect of a tetrodotoxin (TTX)-sensitive sodium conductance on tubular depolarization.

Citations

May 1, 1990·Pflügers Archiv : European journal of physiology·J A HeinyS H Bryant
Jan 1, 1988·Pflügers Archiv : European journal of physiology·N ArispeE Rojas
Aug 1, 1987·Pflügers Archiv : European journal of physiology·R Bournaud, A Mallart
Jan 1, 1985·Progress in Biophysics and Molecular Biology·J CailléO Rougier
Jun 1, 1996·Comparative Biochemistry and Physiology. Part A, Physiology·N Fujishiro, H Kawata
May 18, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·N FujishiroH Kawata
Nov 28, 2007·The Journal of General Physiology·Marino DiFrancoJulio L Vergara
Dec 15, 2010·The Journal of General Physiology·Marino DiFrancoJulio L Vergara
Nov 17, 2010·The Journal of General Physiology·John D LueckRobert T Dirksen
Sep 29, 2011·The Journal of General Physiology·Marino DiFranco, Julio L Vergara
Oct 22, 1986·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J Vergara, M Delay
Oct 4, 2000·Physiological Reviews·O M Sejersted, G Sjøgaard

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