Optical signature of nerve tissue-Exploratory ex vivo study comparing optical, histological, and molecular characteristics of different adipose and nerve tissues

Lasers in Surgery and Medicine
Andrea J R BalthasarArno Lataster

Abstract

During several anesthesiological procedures, needles are inserted through the skin of a patient to target nerves. In most cases, the needle traverses several tissues-skin, subcutaneous adipose tissue, muscles, nerves, and blood vessels-to reach the target nerve. A clear identification of the target nerve can improve the success of the nerve block and reduce the rate of complications. This may be accomplished with diffuse reflectance spectroscopy (DRS) which can provide a quantitative measure of the tissue composition. The goal of the current study was to further explore the morphological, biological, chemical, and optical characteristics of the tissues encountered during needle insertion to improve future DRS classification algorithms. To compare characteristics of nerve tissue (sciatic nerve) and adipose tissues, the following techniques were used: histology, DRS, absorption spectrophotometry, high-resolution magic-angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy, and solution 2D 13 C-1 H heteronuclear single-quantum coherence spectroscopy. Tissues from five human freshly frozen cadavers were examined. Histology clearly highlights a higher density of cellular nuclei, collagen, and cytoplasm in fascicular ner...Continue Reading

References

Oct 29, 2002·FEBS Letters·Bodil Ramstedt, J Peter Slotte
Nov 1, 2003·The Spine Journal : Official Journal of the North American Spine Society·John K Houten, Thomas J Errico
Jan 27, 2006·Anesthesiology·Andrew T Gray
Oct 24, 2007·Analytical and Bioanalytical Chemistry·Venkata Radhakrishna KondepatiJuergen Backhaus
Mar 6, 2008·Microscopy Research and Technique·Federica Di ScipioStefano Geuna
Apr 1, 2009·Spine·Gautam MalhotraMichael Rhee
Jul 10, 2009·Regional Anesthesia and Pain Medicine·Xavier Sala-BlanchAdmir Hadzic
Oct 30, 2009·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·David J KennedyNikolai Bogduk
Mar 23, 2010·Regional Anesthesia and Pain Medicine·Joseph M Neal
Mar 13, 2010·Science·Matthew Paszek, Valerie Weaver
Jul 10, 2010·Journal of Biomedical Optics·Rami NachabéHenricus J C M Sterenborg
Aug 7, 2010·Current Opinion in Anaesthesiology·Jeff GadsdenAdmir Hadzic
Nov 12, 2010·Anesthesiology·James P RathmellBjörn Holmström
Dec 8, 2010·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Naveen S MurthyCurt L Behrns
Jun 10, 2011·Regional Anesthesia and Pain Medicine·Marcus BrynolfBjörn Holmström
Aug 3, 2011·Journal of Biomedical Optics·Adrien E DesjardinsBjörn Holmström
Aug 27, 2011·Current Opinion in Anaesthesiology·Michael J Barrington, Gabriel L Snyder
Dec 6, 2011·Regional Anesthesia and Pain Medicine·Andrea BalthasarMaarten van Kleef
Apr 26, 2012·Regional Anesthesia and Pain Medicine·Andrea BalthasarMicha Sommer
Jan 15, 2013·Current Pain and Headache Reports·Kenneth D Candido, Nebojsa 'nick' Knezevic
May 24, 2013·Journal of Biomedical Optics·Caigang Zhu, Quan Liu
Dec 19, 2013·TheScientificWorldJournal·Abdullah S TerkawiMarcel E Durieux

❮ Previous
Next ❯

Methods Mentioned

BETA
X‐ray
nuclear magnetic resonance
NMR

Software Mentioned

IFAS

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.