PMID: 2500061Jul 1, 1989Paper

Optical spectra and kinetics of reactions of prostaglandin H synthase: effects of the substrates 13-hydroperoxyoctadeca-9,11-dienoic acid, arachidonic acid, N,N,N',N'-tetramethyl-p-phenylenediamine, and phenol and of the nonsteroidal anti-inflammatory drugs aspirin, indomethacin, phenylbutazone, and bromfenac

Archives of Biochemistry and Biophysics
I D MacDonaldH B Dunford

Abstract

A combination of cyclooxygenase activity assays, rapid spectrophotometry and pre-steady-state, steady-state, and transient-state kinetics is used to characterize further the properties of prostaglandin H synthase. 13-Hydroperoxyoctadeca-9-11-dienoic acid is used as oxidizing substrate and the effects of the following compounds are examined: arachidonic acid, N,N,N',N'-tetramethyl-p-phenylenediamine, phenol, diethyldithiocarbamate, and the nonsteroidal anti-inflammatory drugs aspirin, indomethacin, phenylbutazone, and Bromfenac. The order of reactivity of four of these substrates, predominantly with compound II of prostaglandin H synthase, is N,N,N',N'-tetramethyl-p-phenylenediamine greater than phenol greater than indomethacin approximately phenylbutazone. Aspirin exhibits no effect. Arachidonic acid causes inactivation. Diethyldithiocarbamate acts as a reducing substrate for the oxidized forms of prostaglandin H synthase. Bromfenac appears to act both as a protective agent and inhibitor.

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Citations

Aug 30, 2000·Prostaglandins & Other Lipid Mediators·A Tsai, R J Kulmacz
Jul 29, 2008·Journal of Lipid Research·Hyoung-Woo Bai, Bao Ting Zhu
Jun 12, 2003·Chemical Reviews·Carol A Rouzer, Lawrence J Marnett
Jan 1, 1992·Archives of Biochemistry and Biophysics·Y C Hsuanyu, H B Dunford

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