Optical transfer functions of 4Pi confocal microscopes: theory and experiment

Optics Letters
M SchraderT Wilson

Abstract

We measure the point-spread function in the two main configurations of 4Pi confocal microscopy as well as in the traditional confocal arrangement and derive the optical transfer functions from the experimental data. The optical transfer functions are in good agreement with their theoretical counterparts. We find a 3.5- to 5-fold increased axial bandwidth of the 4Pi confocal microscope and hence confirm the enhanced spatial-frequency content of 4Pi images.

References


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Citations

Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Alexander EgnerStefan W Hell
Jul 21, 2010·The Journal of Cell Biology·Lothar SchermellehHeinrich Leonhardt
May 24, 2016·Applied Physics Reviews·P P MathaiS M Stavis
Nov 2, 2001·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·P T SoC Y Dong
Aug 4, 2011·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Eric Le MoalAnne Sentenac
Sep 2, 2006·Applied Optics·David BaddeleyChristoph Cremer
Aug 16, 2006·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·P C PetersE J Harry
Dec 16, 2020·PloS One·Nicholas A W Bell, Justin E Molloy

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