Optical trapping of unilamellar phospholipid vesicles: investigation of the effect of optical forces on the lipid membrane shape by confocal-Raman microscopy

Analytical Chemistry
Daniel P CherneyJoel M Harris

Abstract

Optical trapping of liposomes is a useful tool for manipulating these lipid vesicles for sampling, mechanical testing, spectroscopic observation, and chemical analysis. Through the use of confocal Raman microscopy, this study addresses the effects of optical forces on the structure of unilamellar, dipalmitoylphosphatidylcholine (DPPC) vesicles, both optically trapped in solution and adhered to a coverslip. The energy and forces involved in optical trapping of lipid vesicles were derived in terms of the dielectric contrast between the phospholipid membrane and the surrounding solution; reflection forces at the membrane/water interface were found to be negligible. At optical powers of 9 mW and greater, unilamellar liposomes trapped in bulk solution experience a gradient force sufficiently strong to bend the vesicle membrane, so that a second bilayer from the same vesicle is drawn into the optical trap, with an energy of approximately 6 x 10(-13) erg. For vesicles adhered to a coverslip, the confocal probe can be scanned through the attached vesicle. Optical forces are insufficient to detach the bilayer that is adhered to the glass; however, the upper DPPC bilayer can be manipulated by the optical trap and the shape of the vesicle...Continue Reading

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Citations

Nov 15, 2011·Nano Letters·Poul M Bendix, Lene B Oddershede
Jul 20, 2010·Annual Review of Analytical Chemistry·Daniel P Cherney, Joel M Harris
Apr 5, 2011·Annual Review of Analytical Chemistry·Zachary D Schultz, Ira W Levin
Dec 10, 2014·Journal of Liposome Research·Ellas SpyratouMersini Makropoulou
Mar 31, 2009·Expert Opinion on Drug Delivery·Ellas SpyratouCostas Demetzos
Aug 6, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Jay P Kitt, Joel M Harris
Jul 19, 2012·Analytical Chemistry·Yuewu XiaoMarta Portoles
Aug 27, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Eric M Peterson, Joel M Harris
Mar 6, 2015·Applied Spectroscopy·Jay P KittJoel M Harris
Jan 9, 2019·Nanoscale·Sergei G KruglikJuan M Falcón-Pérez
Feb 14, 2006·Physical Chemistry Chemical Physics : PCCP·Alina FrantescuEberhard Neumann
Oct 17, 2018·Nature Communications·Jelle PendersMolly M Stevens
Jan 23, 2020·Electrophoresis·Adnan MorshedPrashanta Dutta
Mar 19, 2021·Light, Science & Applications·Yuquan ZhangXiaocong Yuan
Jun 15, 2006·Analytical Chemistry·Marian NavratilEdgar A Arriaga

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