Abstract
The biochemical and biomechanical determinants of adhesion between two biological objects following a collision are complex, and may vary from one system to another. We wished to develop an assay in which all the relevant factors, including the components of the solution, the relative orientation and the relative collision velocity, are under the user's control. A new assay is described in which two mesoscale particles are caused to collide using two independently controlled optical tweezers (optically controlled collision, OPTCOL). This assay enables precise examination of the probability of adhesion under biologically relevant conditions. The OPTCOL assay was used to evaluate the probability of adhesion of a single erythrocyte to a single virus-coated microsphere, in the absence and presence of a sialic acidbearing inhibitor. Inhibition constants for the most effective inhibitors could not be measured using other types of assays. The best inhibitor prevented attachment 50 % of the time at a sialic acid concentration of 35 pmol l-1; it is the most potent known inhibitor of attachment of influenza virus to erythrocytes. OPTCOL is a versatile new bioassay for studying dynamic interactions in biochemistry. It offers an approach t...Continue Reading
References
Oct 13, 1992·Biochemistry·N K SauterD C Wiley
Dec 24, 1987·Nature·A AshkinT Yamane
Mar 20, 1987·Science·A Ashkin, J M Dziedzic
Oct 13, 1995·Journal of Medicinal Chemistry·M MammenG M Whitesides
Nov 9, 1995·Nature·J E MolloyD C White
Apr 1, 1994·Antiviral Research·L V MochalovaM N Matrosovich
Jun 1, 1995·Biophysical Journal·E EvansR Merkel
Sep 21, 1995·Nature·T NishizakaK Kinosita
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·K Svoboda, S M Block
May 6, 1994·Science·T T PerkinsS Chu
May 1, 1996·Immunity·P A NegulescuM D Cahalan
Feb 1, 1996·Chemistry & Biology·S K ChoiG M Whitesides
Citations
Sep 27, 2002·Biophysical Journal·Simone KulinKristian Helmerson
Feb 6, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ravi S Kane
May 31, 2008·Nature Methods·Keir C Neuman, Attila Nagy
Apr 9, 2008·Antimicrobial Agents and Chemotherapy·Pamela R HallRichard S Larson
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·A Ashkin
May 12, 2009·Annals of the New York Academy of Sciences·Todd SulchekAleksandr Noy
Oct 23, 1997·Structure·R C Wade
Jan 23, 1999·Biophysical Journal·S HénonF Gallet
Sep 23, 2016·Scientific Reports·Jisu Kim, Jung H Shin
Aug 7, 2007·Chembiochem : a European Journal of Chemical Biology·Eiton KaltgradOla Blixt
May 12, 2005·Cell Motility and the Cytoskeleton·Rachel Patton McCordKaren K Bernd
Apr 4, 2006·Physical Biology·A GhoshD Mathur
Jul 19, 2003·Applied Optics·Erik Fällman, Ove Axner
Dec 23, 2016·Reports on Progress in Physics·Karl Otto Greulich
Dec 2, 2017·Advanced Healthcare Materials·Pranjal NautiyalArvind Agarwal
Nov 25, 2000·Angewandte Chemie·R E HolmlinG M Whitesides
Nov 28, 2020·Ultrasonics·D ZhaoR Marchiano
Jul 6, 2002·The Journal of Organic Chemistry·Alessandro DondoniHarold D Banks