Optically-controlled platforms for transfection and single- and sub-cellular surgery

Biophysics Reviews
Mark VillangcaJesper Glückstad

Abstract

Improving the resolution of biological research to the single-cell or sub-cellular level is of critical importance in a wide variety of processes and disease conditions. Most obvious are those linked to aging and cancer, many of which are dependent upon stochastic processes where individual, unpredictable failures or mutations in individual cells can lead to serious downstream conditions across the whole organism. The traditional tools of biochemistry struggle to observe such processes: the vast majority are based upon ensemble approaches analysing the properties of bulk populations, which means that details of individual constituents is lost. What are required, then, are tools with the precision and resolution to probe and dissect cells at the single-micron scale: the scale of the individual organelles and structures that control their function. In this review, we highlight the use of highly-focused laser beams to create systems which provide precise control and specificity at the single-cell or even single-micron level. The intense focal points generated can directly interact with cells and cell membranes, which in conjunction with related modalities such as optical trapping provide a broad platform for the development of sin...Continue Reading

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Citations

Nov 28, 2017·Scientific Reports·Haichao YuJun Yang
Jul 2, 2019·Biomedical Optics Express·Herbert Schneckenburger
Sep 23, 2016·Light, Science & Applications·Mark Jayson VillangcaJesper Glückstad

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