Optically Detected Magnetic Resonance of Chlorophyll Triplet States in Water-Soluble Chlorophyll Proteins from Lepidium virginicum: Evidence for Excitonic Interaction among the Four Pigments

The Journal of Physical Chemistry. B
Alessandro AgostiniD Carbonera

Abstract

Optically detected magnetic resonance of triplet states populated by photoexcitation in water-soluble chlorophyll proteins (WSCPs) from Lepidium virginicum has been performed using both absorption and fluorescence detection. Well resolved triplet-singlet (T-S) spectra have been obtained and interpreted in terms of electronic interactions among the four chlorophylls (Chls), forming two dimers in the WSCP tetramer. Localization of the triplet state on a single Chl leads to a redistribution of the oscillator strength in the remaining three Chls of the complex. By comparing the spectra with those obtained on a substoichiometric WSCP complex containing only 2 Chls per protein tetramer, we proved that, to interpret the optical spectra of the WSCP fully loaded with 4 Chls, the interactions between the two dimers have to be taken into account and cannot be considered negligible. The results show that the WSCP may well be considered as an ideal model system to study Chl-Chl interactions, also in view of the possibility to modify the number and molecular structure of the bound porphyrin chromophores.

References

Aug 6, 1971·Biochimica Et Biophysica Acta·T MurataE Yakushiji
Jun 19, 2002·Biochemistry·Stefano SantabarbaraDonatella Carbonera
Mar 16, 2006·Journal of the American Chemical Society·Joseph L HughesElmars Krausz
Dec 16, 2006·The Journal of Biological Chemistry·Daisuke HorigomeAkira Uchida
Feb 25, 2009·Photosynthesis Research·Donatella Carbonera
Mar 19, 2014·The Journal of Physical Chemistry. B·Jan AlsterDonatas Zigmantas
Dec 8, 2014·Biochimica Et Biophysica Acta·Stefano SantabarbaraDonatella Carbonera
Apr 10, 2015·The Journal of Physical Chemistry. B·Shigeharu KiharaSergei Savikhin
Feb 26, 2016·Photochemistry and Photobiology·Adam KellRyszard Jankowiak
Apr 22, 2016·Angewandte Chemie·Dominika BednarczykDror Noy
Mar 3, 2017·Biochemistry·Daniel M PalmHarald Paulsen
Jan 24, 2018·The Journal of Physical Chemistry Letters·Alessandro AgostiniDonatella Carbonera

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Citations

Apr 28, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Philipp GirrHarald Paulsen
Mar 11, 2021·Physical Chemistry Chemical Physics : PCCP·Yigal LahavIgor Schapiro
May 22, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alessandro AgostiniDonatella Carbonera
Sep 11, 2019·The Journal of Physical Chemistry. B·Alessandro AgostiniDonatella Carbonera
Sep 23, 2020·Biochimica Et Biophysica Acta. Biomembranes·Philipp Girr, Harald Paulsen

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