Optimal combination of media for primary isolation of Helicobacter pylori from gastric biopsy specimens.

Journal of Clinical Microbiology
R PiccolominiM Neri

Abstract

The aim of the present study was to compare eight media, four nonselective and four selective media, to determine the best combination of media for the primary isolation of Helicobacter pylori. Over a period of 5 months, mucosal antral biopsy specimens were obtained from 222 consecutive dyspeptic patients undergoing endoscopy. Biopsy samples were plated in parallel on all eight media. Egg yolk emulsion agar (EYE), Skirrow's medium, Dent's medium, and modified Thayer-Martin medium were used as selective media; modified chocolate agar (MCHOC), Triptycase soy agar (TSA), brucella agar, and brain heart infusion agar were used as nonselective media. Overall, by using these eight media, H. pylori was recovered from biopsy specimens from 114 of 222 patients, yielding an isolation rate of 51%. Comparison of all possible combinations of the eight media showed that the highest rate of isolation of H. pylori was 100% (114 of 114) with EYE-MCHOC, followed by 96.5% (110 of 114) when EYE-TSA was used. Conversely, it was found that none of the media used alone yielded a 100% rate of recovery (the maximum recovery rate was 95%, which was achieved with EYE). These results indicate that the association of EYE and MCHOC yielded the maximum recove...Continue Reading

References

Apr 1, 1991·Journal of Clinical Microbiology·T U WestblomB R Midkiff
Jan 1, 1991·Journal of Clinical Microbiology·R AnsorgE N Schmid
Jan 1, 1990·Clinical Microbiology Reviews·G E Buck
Jan 1, 1987·American Journal of Clinical Pathology·D E TaylorL D Jewell
Dec 7, 1989·The New England Journal of Medicine·C P DooleyM J Blaser
Feb 1, 1989·Gastroenterology·D Y Graham
Aug 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J C Dent, C A McNulty
May 1, 1988·Journal of Clinical Microbiology·J ParsonnetM J Ferraro
Apr 15, 1985·The Medical Journal of Australia·B J MarshallR J Glancy
Dec 1, 1985·Journal of Clinical Microbiology·F MegraudH Lamouliatte
Feb 1, 1981·Journal of Clinical Microbiology·C M PattonA F Kaufmann
Aug 1, 1995·Journal of Clinical Pathology·C Y HachemD Y Graham

❮ Previous
Next ❯

Citations

Mar 26, 2005·Lasers in Surgery and Medicine·Robert A GanzMichael R Hamblin
Jan 21, 2006·Helicobacter·Adriana Gonçalves OliveiraDulciene Maria Magalhães Queiroz
Feb 3, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R Piccolomini, G Di Bonaventura
Apr 17, 2001·Antimicrobial Agents and Chemotherapy·R PiccolominiM Neri
Feb 19, 2002·Alimentary Pharmacology & Therapeutics·D VairaM Miglioli
Oct 11, 2003·Alimentary Pharmacology & Therapeutics·M NeriF Cuccurullo
Dec 2, 2009·Current Microbiology·Masoumeh DouraghiMarjan Mohammadi
Oct 9, 2003·Alimentary Pharmacology & Therapeutics·H O'Connor, S Sebastian
Jun 18, 2009·Lasers in Surgery and Medicine·Anthony J LemboMichael R Hamblin
Mar 27, 2015·Journal of Clinical Gastroenterology·Hawazen IsmailGareth Jenkins
Apr 13, 2007·Clinical Microbiology Reviews·Francis Mégraud, Philippe Lehours
Jan 9, 2004·Applied and Environmental Microbiology·N F AzevedoM J Vieira
Dec 30, 2017·Toxins·Jesus GuzmanMichel Sauvain
Dec 10, 2003·Journal of Clinical Microbiology·Alba E VegaOlga Puig De Centorbi
Sep 8, 2006·Journal of Clinical Microbiology·Keigo ShibayamaYoshichika Arakawa
Dec 17, 1998·Journal of Clinical Microbiology·R PiccolominiE Pizzigallo
Mar 10, 2007·International Journal of Immunopathology and Pharmacology·G Di BonaventuraM Neri

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Eastern Mediterranean Health Journal = La Revue De Santé De La Méditerranée Orientale = Al-Majallah Al-ṣiḥḥīyah Li-sharq Al-mutawassiṭ
A Al-SulamiH Hunoon
European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
R W van der HulstG N Tytgat
© 2021 Meta ULC. All rights reserved