Optimal conditions and specificity of interaction of a distinct class of nonhistone chromosomal proteins with DNA

T L Thomas, G L Patel


A subclass of nonhistone chromatin proteins with high DNA affinity has been isolated from rat liver. The interaction of the isolated proteins with DNA in vitro was characterized utilizing a nitrocellulose filter binding technique. The temperature, time, concentration, ionic strength, and pH dependence were characterized. Optimal interaction was observed at 0.19 M naCl, pH 7.5 with a protein to DNA ratio of 13 (w/w). Equilibrium and kinetic competition experiments indicated that these proteins interact optimally with A-T rich and single-stranded DNA. The data also suggest that these proteins might affect the helixcoil transiton of DNA.


Jul 14, 1972·Journal of Molecular Biology·B M AlbertsH Delius
Jan 1, 1968·Analytical Biochemistry·N A CummingsH A Sober
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G L Patel, T L Thomas
Jan 1, 1972·Annual Review of Biochemistry·P H Von Hippel, J D McGhee
Jan 1, 1972·Ciba Foundation Symposium·W Gilbert
Sep 20, 1974·Nature·J C WangS Bourgeois
Dec 8, 1972·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·G L Patel
Jan 16, 1973·Biochemistry·H W van den BroekJ Bonner
May 25, 1971·Biochemistry·S L Commerford
Jul 14, 1968·Journal of Molecular Biology·A D Riggs, S Bourgeois
Jul 14, 1968·Journal of Molecular Biology·A D RiggsM Cohn
Apr 25, 1972·Biochemistry·S LevyH A Sober
Jan 27, 1971·Nature: New Biology·R J Clark, G Felsenfeld
Jun 13, 1970·Nature·L J KleinsmithA Carroll
Feb 1, 1965·Journal of Molecular Biology·F W STUDIER


Feb 1, 1977·Archives of Biochemistry and Biophysics·N C KostrabaT Y Wang
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T L Thomas, G L Patel
Jan 1, 1984·Preparative Biochemistry·N DalayE Bermek
Dec 1, 1978·Nucleic Acids Research·J Rzeszowska-WolnyM Chorazy
May 1, 1978·Nucleic Acids Research·L L JagodzinskiJ S Sevall
May 16, 1978·European Journal of Biochemistry·V G CorcesJ Avila
Jan 29, 1982·Biochemical and Biophysical Research Communications·F C BennettL C Yeoman

Related Concepts

Cations, Monovalent
DNA, Double-Stranded
Hydrogen-Ion Concentration
Plasma Protein Binding Capacity

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.