Sep 1, 1976

Optimal conditions for elution of hepatitis B antigen after absorption onto colloidal silica

Journal of Clinical Microbiology
J PillotR G Keros

Abstract

Hepatitis B surface antigen (HBSAg) adsorbed from sera onto colloidal silica could be completely eluted through the use of 0.25% sodium deoxycholate in 0.01 M borax, pH 9.3, at 56 degrees C. The HBSAg recovered in the eluate represented 100% of that present in the original serum, and it was contaminated by only trace amounts of serum proteins (in decreasing amounts: beta-lipoprotein, immunoglobulin G, albumin). This preliminary step greatly facilitates purification of large amounts of HBSAg and provides small volumes of highly concentrated material for subsequent purification by density gradient centrifugation.

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Mentioned in this Paper

Viral Vaccines
Centrifugation, Density Gradient
Hepatitis B Antigens
Process of Absorption
Serum Proteins
Phosphoglycerate Dehydrogenase Activity
Hepatitis B antigen peptide
Blood
Hepatitis B Surface Antigens
Immunoglobulin G [EPC]

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