PMID: 6195175Aug 1, 1983Paper

Optimal conditions for titration of SV40 by the plaque assay method

Journal of Virological Methods
J L Fendrick, L M Hallick

Abstract

The parameters of the Simian Virus 40 (SV40) plaque assay on African green monkey kidney cells were optimized for reproducibility and maximum plaquing efficiency. Plaques were visible as early as 8 days postinfection; maximum titers were obtained with a 10- to 11-day incubation period. Titers read 12-16 days postinfection were not significantly higher than those observed after 10-11 days. Adsorption volumes greater than 0.1 ml/60 mm Petri dish decreased plaque forming units (PFUs) detected. Times greater than 60 min for adsorption of virus to the cell monolayer did not significantly increase the titer; adsorption times less than 60 min resulted in decreased titers. Under standard conditions, 3 ml of overlay medium containing 0.8% agar was applied following virus adsorption and again on days 5 and 10. Concentrations of fetal calf serum (FCS) in the overlay medium of 2.5 to 7.5% gave equal plaque formation. FCS concentrations of 1 and 10% resulted in slightly decreased and increased plaquing efficiencies respectively. Of the reagents tested, agar or agarose containing overlay media produced plaques of maximum number and size. An overlay of methyl cellulose resulted in the same number of plaques, but their size was reduced by appr...Continue Reading

References

Jul 1, 1975·Virology·J SeehaferJ S Colter
Feb 1, 1978·The Journal of General Virology·J SeehaferJ S Colter
Sep 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R M D'Alisa, E L Gershey
Oct 1, 1968·Proceedings of the Society for Experimental Biology and Medicine·H Mirchamsy, F Rapp
May 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·S A Aaronson, G J Todaro
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·V MorhennG M Tomkins
Oct 1, 1981·Journal of Virological Methods·B H RosenbergG E Ungers
Mar 1, 1962·Virology·S STINEBAUGH, J L MELNICK
May 1, 1963·The Journal of Cell Biology·N GRANBOULANW BERNHARD
Jan 22, 1965·Science· RIGGSE H LENNETTE
Nov 1, 1964·Virology·P H BLACKL V CRAWFORD

Citations

Nov 1, 1987·Journal of Virological Methods·J Husson-van VlietP Lemoine
Aug 1, 1988·Journal of Virological Methods·B Kournikakis, J Fildes
Oct 1, 1990·Journal of Virological Methods·G Bushar, J L Sagripanti
Nov 7, 2000·Journal of Virological Methods·S J OrlandoE Gharakhanian
Nov 15, 2000·International Journal of Food Microbiology·J O'MahonyC Hill
Dec 1, 1989·The Journal of Cell Biology·J KartenbeckA Helenius
Jan 29, 2000·Photochemistry and Photobiology·J HirayamaS Sekiguchi

Related Concepts

Adsorption
Cercopithecus tantalus
Cytopathogenic Effect, Viral
Saviosol
Rheumabene
Immune Sera
Bacteriophage Plaque Assay
Simian virus 40
Dextran Sulfate Sodium

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