Abstract
Monoclonal antibodies (MAbs) are widely applied in basic research, medicine, and the pharmaceutical industry. Recently, applications and generations of MAbs have been increasingly attracting attention in many research areas since MAbs could be produced in large quantities with the development of genetic technology and antibody engineering. On the other hand, in recent years, phage display system has been developed for high-throughput isolation and generation of novel MAbs that have high affinity with various antigens. This technology is capable of constructing "Library" containing billions of phage repertoires displaying various antibody fragments, and rapid selection of a specific MAb from this phage library. Additionally, this technology has a great advantage that MAbs can be generated without immunization to animals. However, there are still relatively few reports confirming that useful MAbs can be derived from non-immune antibody libraries. The latter, as undertaken by current methods, seem unable to achieve the high quality required to produce useful MAbs for any desired antigen because cloning of antibody gene from non-immune donors is inefficient. This problem is caused by the fact that their RT-PCR primer sets, PCR cond...Continue Reading
References
Feb 1, 1978·European Journal of Immunology·G KöhlerM J Shulman
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W GerhardH Koprowski
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H GramA S Kang
Aug 15, 1991·Nature·T ClacksonG Winter
Jun 14, 1985·Science·G P Smith
Sep 1, 1996·Nature Medicine·R H BegentL Robson
Feb 14, 1997·Journal of Immunological Methods·A KrebberA Plückthun
Mar 21, 1998·Current Opinion in Biotechnology·A D Griffiths, A R Duncan
Mar 1, 1996·Nature Biotechnology·T J VaughanK S Johnson
Jun 29, 1999·Nature Biotechnology·P S Chowdhury, I Pastan
Dec 22, 1999·Circulation·J C FitchS K Shernan
Mar 15, 2001·The New England Journal of Medicine·D J SlamonL Norton
Feb 6, 2002·Journal of Molecular Biology·Steffen GoletzUwe Karsten
Feb 14, 2003·The New England Journal of Medicine·Filip BaertPaul Rutgeerts
Apr 1, 2003·Nature Biotechnology·Yoko YamamotoTadanori Mayumi
Aug 20, 2003·Circulation·Kenneth W MahaffeyUNKNOWN COMPLY Investigators
Nov 5, 2003·Gastroenterology·Chinyu G Su, Gary R Lichtenstein
Mar 5, 2004·Expert Opinion on Biological Therapy·Maria G Russeva, Gregory P Adams
Citations
Jan 30, 2009·BMC Biotechnology·Potjamas PansriMontarop Yamabhai
Dec 17, 2009·BMC Biotechnology·M Enamul KabirTadazumi Komiyama
Jul 5, 2006·Biological & Pharmaceutical Bulletin·Sunao ImaiShin-ichi Tsunoda
Apr 8, 2010·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takuya YamashitaKazuo Maruyama
Jan 30, 2014·Toxicon : Official Journal of the International Society on Toxinology·Xiao ZhangXianjin Liu
Nov 17, 2012·BMC Biotechnology·Rajesh KumarKalpana Luthra
Aug 11, 2015·Ukrainian biochemical journal·O S OliinykS V Komisarenko
Apr 7, 2012·Journal of Virological Methods·Dongbo SunLi Feng
Jan 23, 2016·Advanced Pharmaceutical Bulletin·Ali Akbar AlizadehSiavoush Dastmalchi
Oct 19, 2010·Protein Expression and Purification·Tao YangBo Niu
Oct 12, 2010·Biomaterials·Sunao ImaiShin-ichi Tsunoda
Sep 8, 2010·Veterinary Immunology and Immunopathology·Andrea BraganzaNicola J Mason
Jan 23, 2010·Journal of Immunological Methods·R SommavillaD Neri
Jan 17, 2009·Journal of Bioscience and Bioengineering·Yoichi KumadaShigeo Katoh
May 21, 2015·Protein Engineering, Design & Selection : PEDS·Keyu LiAnna M Wu
Jun 9, 2006·Biochemical and Biophysical Research Communications·Maki KawamuraShin-ich Tsunoda
Sep 20, 2005·Biochemical and Biophysical Research Communications·Gertrudis RojasJorge V Gavilondo
Aug 30, 2011·Biotechnology Advances·Mohammad Reza SafarnejadStefan Schillberg
Jun 14, 2012·MAbs·Pablo SoteloMaría Carmen Molina
Feb 1, 2013·Blood·Mai YoshikawaShinsaku Nakagawa
Dec 9, 2016·Journal of Occupational and Environmental Medicine·Guang-Hui ZhangWeidong Wu
Sep 28, 2017·International Journal of Molecular Sciences·Gaoxin LeiShuhua Tan
Feb 9, 2019·Preparative Biochemistry & Biotechnology·Samira Pourtaghi-AnvarianSiavoush Dastmalchi
Jun 26, 2009·Molecular Biology Reports·Maneerat KoohapitagtamTheerapol Sirinarumitr
Apr 13, 2017·Frontiers in Microbiology·Abdullah F U H SaeedShihua Wang