Optimal dosage and early intervention of L-ascorbic acid inhibiting K2 Cr2 O7 -induced renal tubular cell damage

Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)
Tzeng-Jih LinI-Jeng Yeh

Abstract

Chromium poisoning can cause renal failure and death. Chromium intoxication may be managed using L-ascorbic acid (vitamin C) therapy. However, the evidence supporting the effectiveness of this treatment is insufficient, and the mechanism of action has not been clarified in renal cells. In this study, our results showed that the optimal regimen of L-ascorbic acid therapy in human epithelial renal proximal tubule cells, HK-2 cells, was 30 μg/mL. Supplementation of L-ascorbic acid with 30 μg/mL and within 8 h of chromium intoxication (K2Cr2O7, Cr6+) was effective to inhibit renal tubular cell damage by blocking generation of free radicals, cell apoptosis, and autophagy. Intracellular chromium concentrations were estimated using electrothermal atomic absorption spectrometry. Treatment of L-ascorbic acid within 8 h of chromium intoxication significantly decreased the entry of chromium into the cells. Moreover, concomitant administration of L-ascorbic acid with repeatedly dosing at 8-hourly intervals had a better protective effect at lower concentration of L-ascorbic acid when compared to single dosing of L-ascorbic acid at an early time point of chromium intoxication. These findings might help physicians develop effective therapy st...Continue Reading

Citations

May 1, 2019·Environmental Science and Pollution Research International·Yali ZhaoDonghai Zhou
Apr 17, 2019·Environmental Science and Pollution Research International·Rida KhanHafiz Abdullah Shakir
May 22, 2020·Environmental Science and Pollution Research International·Shaukat AliMuhammad Adeeb Khan
Dec 14, 2018·Environmental Science and Pollution Research International·Shaukat AliMazhar Ulhaq
Aug 31, 2019·Metabolites·I-Jeng YehChiung-Yu Peng

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