Optimal flip angle for robust practical quantitative NMR measurement using a fixed pulse length

Magnetic Resonance in Chemistry : MRC
Jinhai GaoBing Wang

Abstract

NMR quantification has been traditionally performed by using internal standards. Although methods using external reference in NMR quantification have been developed, the major obstacles in using external referencing method are the measurement deviations associated with changing sample conditions and the requirement of pulse width calibration for every sample in order to compensate these errors. The calibration process is time consuming and in some cases impossible. We developed a quantitative NMR method fixed pulse length (FIXPUL) for all measurements without sample-by-sample calibration. The method is based on the use of an optimal flip angle calibrated for an external standard so that the quantitative errors associated with the pulse width variations are minimized. FIXPUL can be implemented on most basic NMR spectrometers and is robust and easily automated. The method is applicable to a wide range of solution NMR samples in chemistry, biology, and drug research and discovery.

References

Aug 1, 1997·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·L BarantinS Akoka
Jan 3, 2001·Journal of Magnetic Resonance· van der Klink JJ
Feb 24, 2006·Journal of the American Chemical Society·Gerhard Wider, Lars Dreier
Jul 11, 2006·Magnetic Resonance in Chemistry : MRC·Lars Dreier, Gerhard Wider
Aug 12, 2008·Journal of Magnetic Resonance·Fabio ZiarelliMatthew P Augustine
Aug 4, 2009·Journal of Magnetic Resonance·Huaping MoDaniel Raftery
Jul 1, 1999·Analytical Chemistry·S AkokaM Trierweiler

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