Optimal fluorescein-to-protein ratios of bacterial direct fluorescent-antibody reagents.

Journal of Clinical Microbiology
G A HébertR M McKinney

Abstract

A number of bacterial systems were studied with specific direct fluorescent-antibody reagents prepared from rabbit antiserum fractions and having a wide range of fluorescein-to-protein ratios. These systems included Bacteroides, Bordetella, Clostridium, Escherichia, Legionella, Listeria, Salmonella, Shigella, and Streptococcus. For all systems studied, a fluorescein-to-protein ratio of 30 was optimal for conjugates prepared from ammonium sulfate fractions (greater than 75% gamma globulin) and pure immunoglobulin G desorbed from the Sepharose-bound protein A of Staphylococcus aureus. A pepsin digestion procedure is described that yielded the F(ab')2 piece of pure immunoglobulin G; this was labeled and studied at two fluorescein-to-protein ratios.

References

Jun 30, 1975·Annals of the New York Academy of Sciences·D Danielsson, U Forsum
Jan 1, 1976·Journal of Immunological Methods·J W Goding
Oct 1, 1959·Proceedings of the Society for Experimental Biology and Medicine·C W SMITHW C EVELAND

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Citations

Jan 1, 1983·Annals of the New York Academy of Sciences·S Chantler, I Batty
Apr 8, 2015·Journal of Physiology and Biochemistry·Sarita NehraDeepika Saraswat
Nov 1, 1986·Journal of Clinical Microbiology·D S HodgeP E Shewen

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