Optimal strategy for obtaining routine chromosome analysis by using negative fractions of CD138 enriched plasma cells

Cancer Genetics
Veronica OrtegaGopalrao Velagaleti

Abstract

Fluorescence in situ hybridization (FISH) is superior to routine chromosome analysis (RCA) in detecting important prognostic genetic abnormalities in plasma cell dyscrasia (PCD); however, its sensitivity is hampered due to paucity of plasma cells (PC) in whole bone marrow (BM). Studies showed that the abnormality detection rate in enriched plasma cells (EPC) is greater than unselected plasma cells (UPC), but purification techniques are limiting to only FISH when sample volumes are inadequate. Not performing RCA may compromise patient care since RCA is equally important for detecting non-PC related abnormalities when the diagnosis is undefined. To resolve this critical issue, we designed a study where an immuno-magnetic CD138 enriched positive selection was used for FISH while the negative fraction (NF) was used to retrieve other myeloid elements for RCA. Parallel FISH studies were performed using UPC and CD138 EPC, while karyotyping was achieved using whole BM and discarded myeloid elements from the NF. Results showed that the abnormality rate of EPC was doubled compared to UPC for FISH, and CA displayed 100% success rate using the NF. PCD related chromosome abnormalities were confined to whole BM while non-PCD related abnormal...Continue Reading

References

Jul 1, 1995·Cancer Genetics and Cytogenetics·J R SawyerB Barlogie
Sep 25, 2001·Blood·N V SmadjaUNKNOWN Groupe Français de Cytogénétique Hématologique
Mar 3, 2004·Cancer Research·Rafael FonsecaHerve Avet-Loiseau
Feb 14, 2009·Cancer Genetics and Cytogenetics·Olga PozdnyakovaPatricia M Miron
Nov 24, 2011·Mediterranean Journal of Hematology and Infectious Diseases·X PapanikolaouS Z Usmani

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Citations

Jul 25, 2017·Gene Therapy·J TarnowskiS Kili
Feb 18, 2021·Expert Review of Molecular Diagnostics·Federica Zito MarinoRenato Franco

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