PMID: 9430961Feb 12, 1998Paper

Optimal temperature of cardiac myocytes preservation under severely hypoxic status--experimental study of the isolated cardiac myocytes

[Zasshi] [Journal]
H Uchino

Abstract

Purpose of the present study was to evaluate functional and biochemical effects of hypoxia and hypothermia on cardiac myocytes, in order to identify the optimal temperature of cardiac myocyte preservation. Cardiac myocytes isolated from the neonatal rat ventricles (1.5 x 10(6) myocytes/culture flask) were incubated under the severely hypoxic conditions (partial pressure of oxygen was 20 mmHg) for 24 hours at 4 degrees C, 10 degrees C, 15 degrees C, 20 degrees C, 25 degrees C, and 37 degrees C. After each hypoxic incubation, CPK and LDH were measured in the incubation media. The myocytes were then cultured for additional 24 hours at 37 degrees C to evaluate the recovery of the myocyte beating rate. In 4 degrees C and 37 degrees C groups, the myocyte beating rate recovery was markedly low as 0.0% and 34.5% of the control, compared to the beating rate pr or to hypoxia, respectively (p < 0.001). Release of CPK and LDH (mIU/flask) was significantly higher in 4 degrees C (CPK: 197.1, LDH: 1395) and 37 degrees C (CPK: 138.6, LDH: 1201) groups, respectively (p < 0.001). CPK and LDH levels did not significantly increase among four (10 degrees C, 15 degrees C, 20 degrees C, and 25 degrees C) groups. In order to further confirm the optima...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.