Optimal trapping stability of Escherichia coli in oscillating optical tweezers.

Physical Review. E
Amarjeet YadavMario Feingold

Abstract

Single-beam oscillating optical tweezers can be used to trap rod-shaped bacterial cells and align them with their long axis lying within the focal plane. While such configuration is useful for imaging applications, the corresponding imaging resolution is limited by the fluctuations of the trapped cell. We study the fluctuations of four of the coordinates of the trapped cell, two for its center of mass position and two for its angular orientation, showing the way they depend on the trap length and the trapping beam power. We find that optimal trapping stability is obtained when the trap length is about the same as the cell length and that cell fluctuations in the focal plane decrease like the inverse of the trapping power.

References

Sep 1, 1992·Biophysical Journal·K SvobodaS M Block
Jul 1, 1993·Yeast·J A GrimbergenG J Brakenhoff
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·R M Berry, H C Berg
Jan 28, 2003·Biophysical Journal·Erwin J G PetermanChristoph F Schmidt
Sep 21, 2004·Biophysical Journal·Jana JassOve Axner
Sep 4, 2007·Biophysical Journal·Galina ReshesMario Feingold
Jul 23, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·E ItanM Feingold
Aug 8, 2005·Optics Express·Caitriona CreelyDmitri Petrov
May 11, 2010·Journal of Biophotonics·Florian HörnerCornelia Denz
Dec 15, 1987·Applied Optics·T N BuicanJ C Martin
Nov 8, 2016·Light, Science & Applications·Anna S BezryadinaZhigang Chen
Dec 14, 2016·Nature Communications·Robin DiekmannThomas Huser
Sep 30, 2017·Frontiers in Microbiology·Pramod KumarMario Feingold
Dec 15, 2019·Scientific Reports·Zheng ZhangMatthias Heinemann

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