Optimal vitrification protocol for mouse ovarian tissue cryopreservation: effect of cryoprotective agents and in vitro culture on vitrified-warmed ovarian tissue survival

Human Reproduction
Hye Won YoumSeok Hyun Kim

Abstract

What is the optimal vitrification protocol according to the cryoprotective agent (CPA) for ovarian tissue (OT) cryopreservation? The two-step protocol with 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 10 min then 20% EG, 20% DMSO and 0.5 M sucrose for 5 min showed the best results in mouse OT vitrification. Establishing the optimal cryopreservation protocol is one of the most important steps to improve OT survival. However, only a few studies have compared vitrification protocols with different CPAs and investigated the effect of in vitro culture (IVC) on vitrified-warmed OT survival. Some recent papers proposed that a combination of CPAs has less toxicity than one type of CPA. However, the efficacy of different types and concentrations of CPA are not yet well documented. A total of 644 ovaries were collected from 4-week-old BDF1 mice, of which 571 ovaries were randomly assigned to 8 groups and vitrified using different protocols according to CPA composition and the remaining 73 ovaries were used as controls. After warming, each of the eight groups of ovaries was further randomly divided into four subgroups and in vitro cultured for 0, 0.5, 2 and 4 h, respectively. Ovaries of the best two groups among the ei...Continue Reading

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Citations

Feb 2, 2016·Biopreservation and Biobanking·Danielle C BritoJulio C Pieczarka
Feb 2, 2016·Biopreservation and Biobanking·Maryam Akhavan TaheriBita Ebrahimi
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Jul 28, 2021·Histochemistry and Cell Biology·Sarah BeschtaArtur Mayerhofer
Aug 17, 2021·Journal of Assisted Reproduction and Genetics·Yodo SugishitaKutluk Oktay

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