Optimisation and significance of ATP analysis for measuring active biomass in granular activated carbon filters used in water treatment

Water Research
A Magic-Knezev, Dick van der Kooij

Abstract

A method for determining the concentration of active microbial biomass in granular activated carbon (GAC) filters used in water treatment was developed to facilitate studies on the interactions between adsorption processes and biological activity in such filters. High-energy sonication at a power input of 40 W was applied to GAC samples for the detachment of biomass which was measured as adenosine triphosphate (ATP). Modelling of biomass removal indicated that a series of six to eight sonication treatments of 2 min each yielded more than 90% of the attached active biomass. The ATP concentrations in 30 different GAC filters at nine treatment plants in The Netherlands ranged from 25 to 5000 ng ATP cm(-3) GAC, with the highest concentrations at long filter run times and pretreatment with ozone. A similar concentration range was observed in nine rapid sand (RS) filters. ATP concentrations correlated significantly (p<0.05) with total direct bacterial cell counts in each of these filter types, but the median value of the ATP content per cell in GAC filters (2.1 x 10(-8) ng ATP/cell) was much lower than in the RS filters (3.6 x 10(-7) ng ATP/cell). Average biofilm concentrations ranging from 500 to 10(5) pg ATP cm(-2) were calculated ...Continue Reading

References

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Citations

Sep 27, 2013·Applied Microbiology and Biotechnology·G LiuJ C Van Dijk
May 26, 2009·Applied and Environmental Microbiology·Rinske M ValsterDick van der Kooij
Sep 21, 2010·Applied and Environmental Microbiology·Rinske M ValsterDick van der Kooij
Dec 15, 2010·Applied and Environmental Microbiology·Frederik HammesMarco Dignum
Nov 13, 2009·Journal of Industrial Microbiology & Biotechnology·Jean-Baptiste PoitelonMichael S DuBow
Jul 26, 2008·Applied Microbiology and Biotechnology·Juntaek LimSeokhwan Hwang
Feb 13, 2016·Frontiers in Microbiology·Emmanuelle I PrestJohannes S Vrouwenvelder
Sep 11, 2012·Water Research·Sabrina BottonEmile R Cornelissen
Jan 3, 2012·Water Research·W W J M de VetL C Rietveld
Oct 11, 2011·Water Research·Silvana VeltenFrederik Hammes
Sep 29, 2015·Bioresource Technology·Guang-Feng YangLiang Zhu
Oct 22, 2008·Water Research·J S VrouwenvelderM C M van Loosdrecht
Jan 13, 2009·Environmental Research·Jinkeun Kim
Jan 13, 2015·Journal of Endodontics·Kai Soo TanGunnar Bergenholtz
Dec 30, 2014·Water Research·Barbara Siembida-LöschPeter M Huck

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