Optimisation of α-terpineol production by limonene biotransformation using Penicillium digitatum DSM 62840

Journal of the Science of Food and Agriculture
Ya-Nan TaiGang Fan

Abstract

In this study, (R)-(+)-limonene biotransformation using three fungal strains was compared. Penicillium digitatum DSM 62840 was distinguished for its capacity to transform limonene into α-terpineol with high regioselectivity. Growth kinetics in submerged liquid culture and the effects of growth phase and contact time on biotransformation were studied using this strain. Substrate concentration, co-solvent selection, and cultivation conditions were subsequently optimised. The maximum concentration of α-terpineol (833.93 mg L(-1)) was obtained when the pre-culture medium was in medium log-phase by adding 840 mg L(-1) substrate dissolved in ethanol and cultivation was performed at 24 °C, 150 rpm, and pH 6.0 for 12 h. Addition of small amounts of (R)-(+)-limonene (84 mg L(-1)) at the start of fungal log-phase growth yielded a 1.5-fold yield of α-terpineol, indicating that the enzyme was inducible. Among these three strains tested, P. digitatum DSM 62840 was proved to be an efficient biocatalyst to transform (R)-(+)-limonene to α-terpineol. Further studies revealed that the optimal growth phase for biotransformation was in the medium log phase of this strain. The biotransformation represented a wide tolerance of temperature; α-terpine...Continue Reading

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Citations

May 24, 2016·The Journal of Dairy Research·Gerardo CajaChristopher H Knight
Jun 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mateusz KutyłaMariusz Trytek
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li ChenSi-Yi Pan
Jul 20, 2021·Journal of Industrial Microbiology & Biotechnology·Xiao LiSi-Yi Pan
Sep 17, 2021·Journal of Industrial Microbiology & Biotechnology·Beena C LadHal S Alper

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