Optimisation of Ethanol-Reflux Extraction of Saponins from Steamed Panax notoginseng by Response Surface Methodology and Evaluation of Hematopoiesis Effect

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Yupiao HuYin Xiong

Abstract

The present study aims to optimize the ethanol-reflux extraction conditions for extracting saponins from steamed Panax notoginseng (SPN). Four variables including the extraction time (0.5⁻2.5 h), ethanol concentration (50⁻90%), water to solid ratio (W/S, 8⁻16), and times of extraction (1⁻5) were investigated by using the Box-Behnken design response surface methodology (BBD-RSM). For each response, a second-order polynomial model with high R² values (>0.9690) was developed using multiple linear regression analysis and the optimum conditions to maximize the yield (31.96%), content (70.49 mg/g), and antioxidant activity (EC50 value of 0.0421 mg/mL) for saponins extracted from SPN were obtained with a extraction time of 1.51 h, ethanol concentration of 60%, extraction done 3 times, and a W/S of 10. The experimental values were in good consistency with the predicted ones. In addition, the extracted SPN saponins could significantly increase the levels of blood routine parameters compared with the model group (p < 0.01) and there was no significant difference in the hematopoiesis effect between the SPN group and the SPN saponins group, of which the dose was 15 times lower than the former one. It is suggested that the SPN saponins extr...Continue Reading

References

Oct 25, 2000·Journal of Microbiological Methods·J TaubertR G Berger
Dec 4, 2003·Journal of Agricultural and Food Chemistry·Roberto MorelliRoberto Lo Scalzo
Jul 29, 2006·The Journal of Pharmacy and Pharmacology·T B Ng
Jul 19, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li-Chun ZhaoGeng-Liang Yang
Jun 6, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xinsheng WangQiaoli Liang
Jan 21, 2015·International Journal of Biological Macromolecules·Peyman Pakrokh Ghavi
Jun 13, 2017·International Journal of Biological Macromolecules·Marzieh HeydarianMohammad Noshad

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