Optimisation of the extraction and purification of chondroitin sulphate from head by-products of Prionace glauca by environmental friendly processes

Food Chemistry
José Antonio VázquezRicardo I Pérez-Martín

Abstract

The goal of the present work was to optimise the different environmental friendly processes involved in the extraction and purification of chondroitin sulphate (CS) from Prionace glauca head wastes. The experimental development was based on second order rotatable designs and evaluated by response surface methodology combined with a previous kinetic approach. The sequential stages optimised were: (1) the enzymatic hydrolysis of head cartilage catalysed by alcalase (55.7 °C/pH 8.2); (2) the chemical treatment of enzyme hydrolysates by means of alkaline-hydroalcoholic saline solutions (NaOH: 0.54 M, EtOH: 1.17 v, NaCl: 2.5%) to end the protein hydrolysis and to precipitate and selectively redissolve CS versus the peptidic material and (3) the selective purification and concentration of CS and the concomitant protein permeation of extracts which were obtained from previous treatment using ultrafiltration and diafiltration (UF-DF) technologies at two different cut-offs.

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Citations

Mar 28, 2017·Marine Drugs·Antonio Trincone
Feb 28, 2018·Biotechnology and Bioengineering·Zhengxiong ZhouZhen Kang
Mar 28, 2017·Applied Microbiology and Biotechnology·Zhengshan LuoJian Chen
Nov 30, 2018·Journal of Food Science and Technology·Xiaoyan ZhaoHan Sun
Feb 6, 2017·Journal of Molecular Recognition : JMR·Vanessa R A FerreiraA Fernando Silva
Apr 11, 2020·International Journal of Biological Macromolecules·Miriam López-ÁlvarezJosé Antonio Vázquez
Jun 14, 2020·Carbohydrate Polymers·Maha M AbdallahMaria do Rosário Bronze
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Patricia Diaz-RodriguezMariana Landin

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