Optimised Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI-MSI) for the Analysis of Proteins/Peptides Directly from Tissue Sections on a Travelling Wave Ion Mobility Q-ToF

Journal of the American Society for Mass Spectrometry
Mark TowersEmmanuelle Claude

Abstract

Desorption electrospray ionisation mass spectrometry imaging (DESI-MSI) is typically known for the ionisation of small molecules such as lipids and metabolites, in singly charged form. Here we present a method that allows the direct detection of proteins and peptides in multiply charged forms directly from tissue sections by DESI. Utilising a heated mass spectrometer inlet capillary, combined with ion mobility separation (IMS), the conditions with regard to solvent composition, nebulising gas flow, and solvent flow rate have been explored and optimised. Without the use of ion mobility separation prior to mass spectrometry analysis, only the most abundant charge series were observed. In addition to the dominant haemoglobin subunit(s) related trend line in the m/z vs drift time (DT) 2D plot, trend lines were found relating to background solvent peaks, residual lipids and, more importantly, small proteins/large peptides of lower abundance. These small proteins/peptides were observed with charge states from 1+ to 12+, the majority of which could only be resolved from the background when using IMS. By extracting charge series from the 2D m/z vs DT plot, a number of proteins could be tentatively assigned by accurate mass. Tissue imag...Continue Reading

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Citations

Mar 19, 2019·Rapid Communications in Mass Spectrometry : RCM·Øystein SkjaervøTrine Grønhaug Halvorsen
Jul 28, 2020·SLAS Discovery·Richard J A GoodwinJosephine Bunch
Feb 6, 2020·Biochemical Society Transactions·Oliver J Hale, Helen J Cooper
Oct 24, 2020·Mass Spectrometry Reviews·Jialing ZhangLivia S Eberlin
Nov 4, 2020·The Review of Scientific Instruments·Kevin J Zemaitis, Troy D Wood
Feb 20, 2021·Journal of the American Society for Mass Spectrometry·Bin Yan, Josephine Bunch
Mar 5, 2021·Analytical Chemistry·Oliver J Hale, Helen J Cooper
Mar 27, 2021·Analytical and Bioanalytical Chemistry·Lieke LamontTiffany Porta Siegel
Feb 17, 2019·Journal of the American Society for Mass Spectrometry·Roshan JavanshadAndre R Venter
Mar 31, 2019·Journal of the American Society for Mass Spectrometry·Bin YanJosephine Bunch
Nov 24, 2019·Journal of the American Society for Mass Spectrometry·Roshan JavanshadAndre R Venter
Jul 11, 2021·Journal of Separation Science·Wenya HuHonggang Nie
Apr 10, 2020·Analytical Chemistry·Emma K SisleyHelen J Cooper
Jul 23, 2020·Journal of Proteome Research·Matthew Waas, Thomas Kislinger
Feb 23, 2019·Analytical Chemistry·Clara L FeiderLivia S Eberlin
Sep 9, 2020·Analytical Chemistry·Shay VimerMichal Sharon
Aug 24, 2021·Analytical Chemistry·Roch AndrzejewskiAlexandre A Shvartsburg
Sep 19, 2020·Journal of the American Society for Mass Spectrometry·Marcel NiehausJosephine Bunch
Jan 26, 2022·Journal of the American Chemical Society·Emma K SisleyHelen J Cooper

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Methods Mentioned

BETA
acetylation

Software Mentioned

Driftscope
MassLynx
SSP
LESA
LMJ
MaxEnt

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