Optimization and Evaluation of a Chitosan/Hydroxypropyl Methylcellulose Hydrogel Containing Toluidine Blue O for Antimicrobial Photodynamic Inactivation

International Journal of Molecular Sciences
Chueh-Pin ChenChin-Tin Chen

Abstract

Photodynamic inactivation (PDI) combined with chitosan has been shown as a promising antimicrobial approach. The purpose of this study was to develop a chitosan hydrogel containing hydroxypropyl methylcellulose (HPMC), chitosan and toluidine blue O (TBO) to improve the bactericidal efficacy for topical application in clinics. The PDI efficacy of hydrogel was examined in vitro against the biofilms of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). Confocal scanning laser microscopy (CSLM) was performed to investigate the penetration level of TBO into viable S. aureus biofilms. Incorporation of HMPC could increase the physicochemical properties of chitosan hydrogel including the hardness, viscosity as well as bioadhesion; however, higher HMPC concentration also resulted in reduced antimicrobial effect. CSLM analysis further demonstrated that higher HPMC concentration constrained TBO diffusion into the biofilm. The incubation of biofilm and hydrogel was further performed at an angle of 90 degrees. After light irradiation, compared to the mixture of TBO and chitosan, the hydrogel treated sample showed increased PDI efficacy indicated that incorporation of HPMC did improve antimicrobial effect. Finally,...Continue Reading

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Citations

Nov 4, 2016·International Journal of Molecular Sciences·Po-Chun PengChin-Tin Chen
Apr 1, 2017·Applied Microbiology and Biotechnology·Faez Iqbal KhanMd Imtaiyaz Hassan
Jun 27, 2017·Journal of Photochemistry and Photobiology. B, Biology·Hui LiangQingyong Li
Jul 17, 2019·AAPS PharmSciTech·Giovana Maria Fioramonti CalixtoCarla Raquel Fontana
Aug 29, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bhavya KhuranaMathias O Senge
Nov 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yonghai FengMingdong Dong
Jul 28, 2020·Photobiomodulation, Photomedicine, and Laser Surgery·Abdulrahman A BalhaddadMary Anne S Melo
Apr 4, 2021·Polymers·Cai-Ling KeChing-Hsuan Lin

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Methods Mentioned

BETA
confocal microscopy
fluorescence imaging
biopsies

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