Optimization and evaluation of surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid.

Proteome Science
Nelson GuerreiroStéphane Charmont

Abstract

Cerebrospinal fluid (CSF) potentially carries an archive of peptides and small proteins relevant to pathological processes in the central nervous system (CNS) and surrounding brain tissue. Proteomics is especially well suited for the discovery of biomarkers of diagnostic potential in CSF for early diagnosis and discrimination of several neurodegenerative diseases. ProteinChip surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is one such approach which offers a unique platform for high throughput profiling of peptides and small proteins in CSF. In this study, we evaluated methodologies for the retention of CSF proteins < 20 kDa in size, and identify a strategy for screening small proteins and peptides in CSF. ProteinChip array types, along with sample and binding buffer conditions, and matrices were investigated. By coupling the processing of arrays to a liquid handler reproducible and reliable profiles, with mean peak coefficients of variation < 20%, were achieved for intra- and inter-assays under selected conditions. Based on peak m/z we found a high degree of overlap between the tested array surfaces. The combination of CM10 and IMAC30 arrays was sufficient to represent between 80-90...Continue Reading

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Citations

Sep 7, 2007·Proteome Science·Toni WhistlerSuzanne D Vernon
Jul 19, 2006·Diagnostic Pathology·Shaheen Emmanuel Lakhan
Aug 12, 2006·Expert Review of Proteomics·Raj S KasthuriGary L Nelsestuen
May 26, 2007·The Journal of Pharmacy and Pharmacology·Fleur L MoseleyGavin Brooks
Feb 17, 2019·Nanomaterials·Pawel Pomastowski, Boguslaw Buszewski
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna Bodzon-KulakowskaJerzy Silberring
Mar 22, 2007·Journal of Proteome Research·Lars EkbladCharlotte Bratt

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Methods Mentioned

BETA
chips
chip
PCA
immunodepletion
proteomic profiling

Software Mentioned

ProteinChips
Ciphergen Express
SPA
CHCA
SIMCA

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