Optimization and Structural Stability of Gold Nanoparticle-Antibody Bioconjugates

ACS Omega
Robert T BuschErick S Vasquez

Abstract

Gold nanoparticles (AuNPs) bound with biomolecules have emerged as suitable biosensors exploiting unique surface chemistries and optical properties. Many efforts have focused on antibody bioconjugation to AuNPs resulting in a sensitive bioconjugate to detect specific types of bacteria. Unfortunately, bacteria thrive under various harsh environments, and an understanding of bioconjugate stability is needed. Here, we show a method for optimizing Listeria monocytogenes polyclonal antibodies bioconjugation mechanisms to AuNPs via covalent binding at different pH values, from 2 to 11, and 2-(N-morpholino)ethanesulfonic acid (MES), 3-(N-morpholino)propanesulfonic acid, NaOH, HCl conditions. By fitting Lorentz curves to the amide I and II regions, we analyze the stability of the antibody secondary structure. This shows an increase in the apparent breakdown of the antibody secondary structure during bioconjugation as pH decreases from 7.9 to 2. We find variable adsorption efficiency, measured as the percentage of antibody adsorbed to the AuNP surface, from 17 to 27% as pH increases from 2 to 6 before decreasing to 8 and 13% at pH 7.9 and 11, respectively. Transmission electron microscopy (TEM) analysis reveals discrepancies between siz...Continue Reading

References

Jan 29, 1988·Biochimica Et Biophysica Acta·W K Surewicz, H H Mantsch
Nov 7, 1999·Journal of Colloid and Interface Science·C E GiacomelliW Norde
Sep 21, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Scott H BrewerStefan Franzen
Jan 26, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Li ShangShaojun Dong
Aug 10, 2007·Acta Biochimica Et Biophysica Sinica·Jilie Kong, Shaoning Yu
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Oct 24, 2009·Environmental Science & Technology·Rute F DomingosKevin J Wilkinson
Jun 12, 2010·Environmental Science & Technology·Zhaoxia JiJeffrey I Zink
Jul 7, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Bhuvnesh BhartiGerhard H Findenegg
Jul 7, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Dorota Bartczak, Antonios G Kanaras
Nov 17, 2011·Nature Chemical Biology·Nicholas Stephanopoulos, Matthew B Francis
Dec 3, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Kanwarjeet Kaur, James A Forrest
Sep 11, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Lucia MarsichValter Sergo
Jan 3, 2013·Advanced Drug Delivery Reviews·Jose-Maria MontenegroWolfgang J Parak
Mar 22, 2013·Journal of the Royal Society, Interface·Daniel B ThomasOdile Madden
Nov 13, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Stefano P BoulosCatherine J Murphy
Jun 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Raluca M FratilaJesús M de la Fuente
Aug 16, 2014·Physical Chemistry Chemical Physics : PCCP·Mritunjoy MaityNakul C Maiti
Jul 25, 2015·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Kumudu SiriwardanaDongmao Zhang
Sep 15, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Suprit DeolYoung-Seok Shon
Jan 12, 2016·ACS Nano·Sergio Dominguez-MedinaStephan Link
Nov 9, 2016·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Ailin WangNicholas C Fitzkee
Jun 14, 2011·Nanomaterials·Pooja M TiwariShree R Singh

❮ Previous
Next ❯

Citations

Feb 7, 2021·Scientific Reports·Célia AribMarc Lamy de la Chapelle
Feb 27, 2021·Journal of Immunoassay & Immunochemistry·Younes SbihiAbdelghani Iddar
Mar 5, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Susan Farfán-CastroSergio Rosales-Mendoza
Feb 13, 2021·Chemistry : a European Journal·Viktoriya Sokolova, Matthias Epple
Apr 12, 2021·Journal of Pediatric Surgery·You-Sheng LinKuen-Song Lin
May 4, 2021·Journal of the American Chemical Society·Alexandra V RamseyMatthew B Francis
Apr 30, 2021·Biotechnology and Bioengineering·Kirk RoffiJacob Pantazis
May 27, 2021·ACS Biomaterials Science & Engineering·Larry O'ConnellYoann Roupioz
Jun 15, 2021·Frontiers in Plant Science·Sunny AhmarKi-Hung Jung
Jul 30, 2021·Journal of Hazardous Materials·Lu ZengChuanlai Xu

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensor
biosensors
dynamic light scattering
protein assay
surface plasmon resonance
Transmission electron microscopy

Software Mentioned

ImageJ
OriginPro
OMNIC Suite

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.