Optimization and validation of Mycobacterium marinum-induced adult zebrafish model for evaluation of oral anti-tuberculosis drugs

International Journal of Mycobacteriology
Jonnalagadda Padma SrideviDharmarajan Sriram

Abstract

Mycobacterium marinum has emerged as a suitable species for induction of tuberculosis-like disease in zebrafish, and various zebrafish models (larval and adult) for drug screening have been proposed in the literature. It is believed that an adult zebrafish model is more useful in drug screening because, apart from assessment of efficacy, one can obtain data on dosage, pharmacokinetics and overall health improvement. This study suggests a simple, cost-effective and resource-efficient protocol for screening of anti-tuberculosis drugs. The parameters used for assessment of infection as well as anti-bacterial response were: (a) bacterial count; and (b) body weight change. An optimization study was conducted to establish the concentration of bacteria required to produce a reproducible phenotype of tuberculosis (TB). A negative control (Amoxicillin) and anti-mycobacterial drugs (Isoniazid, Rifampicin, Moxifloxacin, Ethambutol and Isoniazid+Rifampicin) were used for validation of the protocol. All the drugs were administered orally. An intra-peritoneal inoculation of 0.75million bacteria/fish was optimized for the model. All the anti-tuberculosis drugs showed efficacy in this model, whereas the negative control did not show any signs ...Continue Reading

References

Aug 28, 1997·The New England Journal of Medicine·L Ramakrishnan
Mar 4, 2006·Microbes and Infection·JoAnne L Flynn
Feb 27, 2008·Cellular Microbiology·David M Tobin, Lalita Ramakrishnan
Jan 27, 2009·Infection and Immunity·Anne E ClatworthyDeborah T Hung
Feb 28, 2009·PLoS Pathogens·Rose L SzabadyRodney A Welch
Jan 15, 2010·Journal of Visualized Experiments : JoVE·Roger Y Tsien
Mar 24, 2010·Infection and Immunity·Chun-Cheih ChaoYung-Jen Chuang
Jun 10, 2010·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·W AldertonI Gardner
Sep 14, 2010·Journal of Visualized Experiments : JoVE·Mary D KinkelVictoria E Prince
Mar 11, 2011·PloS One·Ralph CarvalhoHerman P Spaink
Apr 2, 2011·International Journal of Ayurveda Research·Manish Kumar GoelJugal Kishore
Jul 31, 2012·Cell Reports·Kevin TakakiLalita Ramakrishnan
Jan 29, 2013·Journal of Pharmacological and Toxicological Methods·Girish Hari ChaudhariPushkar Kulkarni
Mar 20, 2014·Cold Spring Harbor Symposia on Quantitative Biology·Lalita Ramakrishnan

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Citations

Mar 15, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jonnalagadda Padma SrideviDharmarajan Sriram
Nov 1, 2016·The Journal of Antimicrobial Chemotherapy·J P DaltonS Wiles
Jan 22, 2019·Critical Reviews in Microbiology·Ghada Bouz, Nada Al Hasawi
Aug 26, 2017·ILAR Journal·Yvonne M BradfordMonte Westerfield
Apr 3, 2021·Frontiers in Cellular and Infection Microbiology·Hee-Jeong YangLaura E Via
Apr 8, 2017·Microbiology Spectrum·Alexandra AubryEmmanuelle Cambau

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