Optimization and validation of RP-HPLC-UV method with solid-phase extraction for determination of buparvaquone in human and rabbit plasma: application to pharmacokinetic study

Biomedical Chromatography : BMC
Gantala VenkateshV Navaratnam

Abstract

A simple, sensitive and specific reversed-phase high-performance liquid chromatographic method with UV detection at 251 nm was developed for quantitation of buparvaquone (BPQ) in human and rabbit plasma. The method utilizes 250 microL of plasma and sample preparation involves protein precipitation followed by solid-phase extraction. The method was validated on a C18 column with mobile phase consisting of ammonium acetate buffer (0.02 m, pH 3.0) and acetonitrile in the ratio of 18:82 (v/v) at a flow rate of 1.1 mL/min. The calibration curves were linear (correlation coefficient>or=0.998) in the selected range. The method is specific and sensitive with limit of quantitation of 50 ng/mL for BPQ. The validated method was found to be accurate and precise in the working calibration range. Stability studies were carried out at different storage conditions and BPQ was found to be stable. Partial validation studies were carried out using rabbit plasma and intra- and inter-day precision and accuracy were within 7%. This method is simple, reliable and can be routinely used for preclinical pharmacokinetic studies for BPQ.

References

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Citations

Jul 16, 2011·International Journal of Analytical Chemistry·Mohammed AkkbikFasihuddin Badruddin Ahmad
Jan 15, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Biyang DengXi Ning
Feb 9, 2010·Drug Development and Industrial Pharmacy·Gantala VenkateshV Navaratnam
Dec 15, 2010·Biomedical Chromatography : BMC·Prashant Laxman KoleRavi Sheshala
Jan 28, 2012·Experimental Parasitology·Juliana Q ReimãoAndré G Tempone
Oct 1, 2010·Pharmaceutical Methods·Saurabh PandeyRuchi Tiwari
May 16, 2018·Molecular Pharmaceutics·Lindsay SmithAikaterini Lalatsa

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