Optimization considerations for the purification of alpha1-antitrypsin using silica-based ion-exchange adsorbents in packed and expanded beds

Biotechnology Progress
G M FinetteM T Hearn

Abstract

The influences of the fluid superficial velocity, sample concentration, loading volume, and wash cycle on the recovery and corresponding purification factors for alpha1-antitrypsin [syn. alpha1-proteinase inhibitor (alpha1-PI)] from crude mixtures of human plasma proteins were investigated using packed and expanded beds of DEAE-Spherodex LS. As part of this study, the effect of fluid superficial velocity on the bed dispersion number (Dv) and dispersion coefficient (D) for this adsorbent in expanded beds was determined with feedstocks containing human serum albumin (HSA), the most abundant of the contaminating proteins in human plasma protein preparations used for the isolation of alpha1-PI. When multicomponent protein feedstocks prepared from human plasma were examined with DEAE-Spherodex LS, reduced chromatographic productivity was observed for alpha1-PI as the extent of column utilization and the superficial velocity were increased, yet the opposite trend was evident for HSA. In particular, higher adsorption capacities and recoveries were obtained for alpha1-PI at lower fluid superficial velocities with both packed and expanded bed conditions. These findings indicate that for process scale purifications of alpha1-PI from mult...Continue Reading

Citations

Feb 16, 2000·Journal of Chromatography. a·F B AnspachW D Deckwer
Oct 19, 2002·Journal of Biotechnology·P E CruzM J T Carrondo
Sep 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Simona ViglioJan Stolk

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