Optimization of β-mannanase production by Bacillus subtilis US191 using economical agricultural substrates.

Biotechnology Progress
Monia BlibechHichem Chouayekh

Abstract

The Bacillus subtilis US191 strain producing highly thermostable β-mannanase was previously selected as potential probiotic candidate for application as feed supplement in poultry industry. Initially, the level of extracellular β-mannanase production by this strain was 1.48 U ml-1 . To improve this enzyme titer, the present study was undertaken to optimize the fermentation conditions through experimental designs and valorization of agro-industrial byproducts. Using the Plackett-Burman design, in submerged fermentation, a set of 14 culture variables was evaluated in terms of their effects on β-mannanase production. Locust bean gum (LBG), soymeal, temperature, and inoculum size were subsequently optimized by response surface methodology using Box-Behnken design. Under optimized conditions (1 g L-1 LBG, 8 g L-1 soymeal, temperature of 30°C and inoculum size of 1010  CFU ml-1 ), a 2.59-fold enhancement in β-mannanase titer was achieved. Next, to decrease the enzyme production cost, the effect of partial substitution of LBG (1 g L-1 ) by agro-industrial byproducts was investigated, and a Taguchi design was applied. This allowed the attaining of a β-mannanase production level of 8.75 U ml-1 in presence of 0.25 g L-1 LBG, 5 g L-1 of c...Continue Reading

References

Jun 9, 2009·Bioscience, Biotechnology, and Biochemistry·Salwa KarbouneSelim Kermasha
Feb 9, 2012·Applied Microbiology and Biotechnology·Prakram Singh ChauhanNaveen Gupta
Jun 15, 2012·Journal of Agricultural and Food Chemistry·Li-Jung YinShann-Tzong Jiang
Jan 15, 2013·The American Journal of Bioethics : AJOB·Zubin Master, David B Resnik
Jan 1, 1994·World Journal of Microbiology & Biotechnology·N S MendozaL M Joson
May 29, 2015·Biotechnology, Biotechnological Equipment·Ebaa Ebrahim El-SharounyAbeer Abd El-Aal El-Gayar
Nov 12, 2016·Biotechnology Advances·Praveen Kumar Srivastava, Mukesh Kapoor
Mar 7, 2017·Journal of Agricultural and Food Chemistry·Yafeng SongDawei Zhang
Jul 27, 2018·International Journal of Biological Macromolecules·Saumya SinghShailendra Kumar Arya
Aug 14, 2019·Biotechnology and Applied Biochemistry·Monia BlibechHichem Chouayekh

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Citations

Jan 2, 2021·Frontiers in Bioengineering and Biotechnology·Aneesa Dawood, Kesen Ma

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