Optimization of γ-polyglutamic acid synthesis using response surface methodology of a newly isolated glutamate dependent Bacillus velezensis Z3

International Microbiology : the Official Journal of the Spanish Society for Microbiology
Ruiyan ZhuJiayu Liu

Abstract

A new glutamate-dependent γ-polyglutamic acid (γ-PGA) producer Z3 isolated from soil samples in Daxinganling forest region of China was identified, and its optimal medium components were investigated using response surface methodology. Strain Z3 was identified as Bacillus velezensis by physiology and biochemistry and 16S rDNA sequence analysis. This is the first report of glutamate-dependent B. velezensis with the ability to synthesize γ-PGA. Then, the optimum γ-PGA yield (5.58 g/L) was achieved with glutamate 86 g/L, glucose 36 g/L, yeast extract powder 5.5 g/L, and NaH2PO4 7.5 g/L. Furthermore, activities of enzymes participating in glutamate synthesis were assessed, and the results showed that lower ketoglutaric dehydrogenase activity (KGDH) and higher glutamate dehydrogenase activity (GDH) resulted in higher γ-PGA yield. Identification of glutamate-dependent γ-PGA producer named B. velezensis Z3 enriches microbiological resources with γ-PGA-producing capacity. B. velezensis optimization of nutrients and analysis of enzymes activities will not only help to increase γ-PGA productivity but also to understand the γ-PGA synthesis mechanism in B. velezensis Z3.

References

Oct 1, 1994·International Journal of Biological Macromolecules·G A BirrerR A Gross
Oct 1, 1995·International Journal of Biological Macromolecules·A M Cromwick, R A Gross
Jan 10, 2002·Applied Microbiology and Biotechnology·M AshiuchiH Misono
Jul 29, 2003·Biotechnology Letters·Andrew Richard, Argyrios Margaritis
Nov 25, 2003·Biotechnology Letters·Ing-Lung ShihYi-Yuan Sau
Nov 10, 2005·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Moon-Hee SungMakoto Ashiuchi
Mar 17, 2007·Critical Reviews in Biotechnology·Joerg M Buescher, Argyrios Margaritis
Aug 1, 2008·Applied Biochemistry and Biotechnology·Jie ChenPeilin Cen
Aug 13, 2008·Applied Biochemistry and Biotechnology·Qun WuJun Yao
Nov 14, 2008·Applied Biochemistry and Biotechnology·Ishwar B Bajaj, Rekha S Singhal
Jun 2, 2009·Canadian Journal of Microbiology·Thomas CandelaAgnès Fouet
Oct 30, 2009·Applied Biochemistry and Biotechnology·Jun YaoPingkai Ouyang
Mar 8, 2011·Bioresource Technology·Ishwar Bajaj, Rekha Singhal
Mar 26, 2011·Applied Biochemistry and Biotechnology·Qun WuPingkai Ouyang
Jan 31, 2013·Applied Microbiology and Biotechnology·Wei ZengZhiqun Liang
Jan 1, 1997·Bioscience, Biotechnology, and Biochemistry·Y OgawaY Tahara
Feb 10, 2017·Microbial Cell Factories·Wencheng YuNing He
Sep 7, 2017·World Journal of Microbiology & Biotechnology·Sarote SirisansaneeyakulYusuf Chisti

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