Optimization of a mouse locomotor rating system to evaluate compression-induced spinal cord injury: correlation of locomotor and morphological injury indices

Journal of Neurosurgery. Spine
Yuesheng LiJoel Linden

Abstract

Due to the usefulness of mouse genetics, there is a need to improve procedures for producing and assessing spinal cord injury (SCI) in mice. The authors describe an improved locomotor scoring system for evaluating SCI. The modified Basso-Beattie-Bresnahan (mBBB) scoring system for mice is compared with existing procedures as well as histological SCI criteria. Mice were subjected to SCI by placing a weight on the cord at T-12 for 5 to 15 minutes after laminectomy to produce spinal cord ischemia. Injury was assessed using mBBB scoring that incorporates elements of the rat BBB and the mouse motor function scoring systems that are best suited for precisely assessing mouse SCI. The mBBB score was found to be more discriminating than the inclined plane test, and in the authors' laboratory it had a significantly lower coefficient of variation than the Basso mouse scale score. The mBBB score is well correlated with sparing of white matter as assessed by eriochrome cyanine staining of myelin. Weight placement at T-12 in the mouse causes reproducible SCI. A new mBBB scoring system is useful for accurately assessing locomotor dysfunction following SCI in mice and is well correlated with histological assessment of spinal cord white matter.

References

Jul 1, 1976·Acta Radiologica: Diagnosis·L Tveten
Jan 1, 1985·Central Nervous System Trauma : Journal of the American Paralysis Association·A R Blight
Feb 1, 1995·Journal of Neurotrauma·D M BassoJ C Bresnahan
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·A W WamilC G Hellerqvist
Sep 8, 1999·Journal of Vascular Surgery·D Rosenthal
Jan 8, 2000·Stroke; a Journal of Cerebral Circulation·L Lang-LazdunskiW D Dietrich
Apr 25, 2000·Journal of Neurotrauma·L B JakemanB T Stokes
Aug 5, 2000·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·L Lang-LazdunskiM Lazdunski
Oct 18, 2001·The Annals of Thoracic Surgery·D C CassadaJ A Kern
Mar 27, 2003·Journal of Neurosurgical Anesthesiology·Takanori SakamotoNoboru Konishi
Dec 1, 1953·A.M.A. Archives of Neurology and Psychiatry·I M TARLOVS VITALE
Aug 7, 2004·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Loïc Lang-LazdunskiChris Rogers
Aug 18, 2004·The Journal of Surgical Research·T Brett ReeceJohn A Kern
Jan 25, 2005·Journal of Neurotrauma·Christie Engesser-CesarCarl W Cotman

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Citations

Nov 6, 2009·Journal of Neurotrauma·Jason E BeareScott R Whittemore
Apr 8, 2011·Annual Review of Neuroscience·Serge Rossignol, Alain Frigon
Mar 2, 2010·Neuroscience Research·Ahdeah Pajoohesh-GanjiAlan I Faden
Jun 19, 2007·Journal of Neuroscience Methods·H B LeveneR F Tuma
Jun 27, 2008·Journal of Neuroscience Methods·Howard B LeveneJack I Jallo
Jul 24, 2012·The American Journal of Pathology·Yona GoldshmitAlice Pébay
Mar 3, 2018·Journal of Neurotrauma·Yona GoldshmitAngela Ruban

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BETA
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Software Mentioned

GraphPad Prism
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