Optimization of alkaline extraction of polysaccharides from Ganoderma lucidum and their effect on immune function in mice.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Sheng-Quan HuangZheng-Xiang Ning

Abstract

Response surface methodology was employed to optimize the conditions for alkaline extraction of polysaccharides from Ganoderma lucidum. The results indicated that the optimum conditions were an extraction temperature of 60.1 degrees C, an extraction time of 77.3 min, a sodium hydroxide (NaOH) concentration of 5.1% and a substrate/liquid ratio of 1:21.4. Immunological assays results have shown that the alkaline soluble polysaccharides have no noticeable effects on monocyte phagocytosis and immune organ (spleen, thymus) weight of immunocompromised mice at the tested dosages. However, they could restore delayed type hypersensitivity reaction to dinitrofluorobenzene (DFNB), hemolysis antibody levels at the three doses applied, and improve the natural killer cell activity at the high-dose and medium dose.

References

Mar 17, 1997·International Journal of Cancer. Journal International Du Cancer·S Y WangC K Ho

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Citations

Jan 18, 2013·International Journal of Food Sciences and Nutrition·Anita KlausLeo J L D Van Griensven
Sep 25, 2015·International Journal of Biological Macromolecules·Napassorn PeasuraSorada Wanlapa
Jul 19, 2011·International Journal of Biological Macromolecules·Jingjuan MengZaifu Liang
Aug 26, 2014·International Journal of Biological Macromolecules·Yongjun KanJinzhong Wu
Jan 25, 2017·Scientia pharmaceutica·Yuliya ProkopenkoVictoriya Georgiyants
Oct 11, 2017·Nutrients·Ahsan HameedYuanda Song
Sep 6, 2017·The Journal of Pharmacy and Pharmacology·Somanjana Khatua, Krishnendu Acharya
Jul 10, 2019·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·B Çelik, H Özparlak
Feb 9, 2018·Carbohydrate Polymers·Christian Winther WoldKari Tvete Inngjerdingen

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Methods Mentioned

BETA
scanning electron microscopy
flow cytometry

Software Mentioned

SPSS
Design
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