Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk

AMB Express
Nuntawat Khat-UdomkiriChaiyavat Chaiyasut

Abstract

Rice husk (RH) is the major agricultural waste obtained during rice hulling process, which can be a sustainable source of xylooligosaccharide (XOS). The current study deals with the production of XOS from Thai rice husk using alkaline pretreatment and enzyme hydrolysis method. The response surface methodology consisted of central composite design and Box-Behnken design was employed to achieve the maximum response in alkaline pretreatment and XOS production, respectively. The optimum conditions for alkaline pretreatment to recover maximum xylan yield were 12-18% of alkaline concentration, the temperature at 110-120 °C, and steaming time for 37.5-40 min. The FTIR results suggested that the extracted sample was the xylan fraction. The maximum XOS production of 17.35 ± 0.31 mg XOS per mL xylan was observed in the run conditions of 6.25 mg enzyme per g xylan, 9 h of incubation time, and 5% of xylan. The results revealed that the xylan extracted from RH by using an effective base couple with the steam application and the enzymatic hydrolysis help to maximize the yield of XOS, which can be further used in functional foods and dietary supplements.

References

Jun 1, 1995·The Journal of Nutrition·G R Gibson, M B Roberfroid
Apr 11, 2008·Applied Biochemistry and Biotechnology·Denise S RuzeneJosé A Teixeira
Feb 13, 2009·Carbohydrate Research·Ozlem AkpinarSeyda Bostanci
Jan 13, 2010·Applied Biochemistry and Biotechnology·Michel BrienzoAdriane M F Milagres
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Dec 18, 2013·Biotechnology and Bioengineering·Xiadi GaoCharles E Wyman
Jun 4, 2015·International Journal of Biological Macromolecules·D Gowdhaman, V Ponnusami

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