Optimization of an in vitro assay to detect Streptococcus equi subsp. equi

Veterinary Microbiology
Ashley G BoyleShelley C Rankin

Abstract

Streptococcus equi is the etiologic agent of a highly infectious upper respiratory disease of horses known as strangles. Bacterial culture methods and polymerase chain reaction (PCR) of nasopharyngeal washes and guttural pouch lavages are used routinely to test clinical and carrier animals for the presence of S. equi but no definitive or gold standard test method has been shown to be optimal. We hypothesized that (i) a flocked swab submerged in ten-fold serial dilution suspensions of S. equi prepared in 0.9% NaCl would detect more colony forming units (CFU) than a rayon swab when used to inoculate a blood agar plate, (ii) centrifugation of a 1 ml aliquot of each suspension would improve the limit of detection (LOD) by bacterial culture and PCR compared to the culture or PCR of submerged swab samples, (iii) PCR of the centrifuged samples from each suspension would be more sensitive than aerobic culture alone, and (iv) PCR of a 1 ml aliquot directly from a sample would be more sensitive than PCR of a sample following submersion of a flocked swab in 1 ml saline. Using 7 ten-fold serial dilutions of S. equi in 0.9% NaCl, the LOD for 4 bacterial culture methods and 3 PCR methods were compared. The LOD of direct PCR and flocked swab ...Continue Reading

References

Apr 1, 1990·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E BingenH Mathieu
Nov 22, 1997·The Veterinary Record·J F Timoney, S C Artiushin
Sep 26, 2008·Diagnostic Microbiology and Infectious Disease·Kenneth G Van HornDenise Sebeck
Nov 18, 2008·Inhalation Toxicology·Ambrogio FassinaMassimo Montisci
Jul 17, 2009·Journal of Clinical Microbiology·David M GoldfarbFrancis Chan
Sep 6, 2011·Annals of Clinical Microbiology and Antimicrobials·Robert SlingerFrancis Chan

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Citations

Dec 19, 2015·Journal of Veterinary Internal Medicine·A G BoyleH Wheeler-Aceto
Sep 29, 2015·Research in Veterinary Science·Guido CordoniRoberto M La Ragione
Jan 21, 2015·The Veterinary Clinics of North America. Equine Practice·Martha Mallicote
Nov 1, 2015·Journal of the American Veterinary Medical Association·Lauren R DuffeeAshley G Boyle

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