Optimization of an MCF7-E3 Cell Proliferation Assay and Effects of Environmental Pollutants and Industrial Chemicals

Toxicology in Vitro : an International Journal Published in Association with BIBRA
D DesaulniersW G Foster

Abstract

Environmental contaminants might adversely affect human health by acting as endocrine disruptors and thus need to be identified. Our objective was to optimize the MCF7 cell proliferation assay to screen industrial chemicals for potential oestrogenic effects. Growth conditions, performance of the clone E3 and WT-MCF7 cells and five methods to derive proliferation indices were compared. The E3 cells were further characterized by testing the effects of transforming growth factorbeta (TGFbeta), epidermal growth factor (EGF), insulin, testosterone, the anti-oestrogen ICI 164,384 (ICI) and environmental contaminants with known oestrogenic potential. Industrial chemicals with unknown oestrogenic effects were then tested. As expected, induction of proliferation by estradiol-17beta (E2) was greater and less variable using the clone E3. To generate proliferation indices, the alamarBlue assay had a sensitivity comparable to that of [(3)H]thymidine incorporation ((3)H-TI). The E3 cells were not responsive to EGF (0-100 ng/ml) or insulin (0-313 ng/ml) but their proliferation was decreased (P<0.05) by TGFbeta (45 ng/ml) and testosterone (10(-8)m), which might be typical of highly oestrogen-responsive MCF7 cells. ICI (5x10(-7)m) inhibited the...Continue Reading

References

Sep 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T E WieseS C Brooks
Aug 1, 1991·Journal of Toxicology and Environmental Health·M J Mac, C C Edsall
Jul 1, 1985·Journal of Steroid Biochemistry·A M Soto, C Sonnenschein
Nov 1, 1970·Journal of Agricultural and Food Chemistry·J Bitman, H C Cecil
Mar 1, 1969·Toxicology and Applied Pharmacology·R M WelchA H Conney
Jan 1, 1983·American Journal of Industrial Medicine·W H Bulger, D Kupfer
Jan 1, 1983·Environmental Mutagenesis·R K HindererD J Brusick
Feb 1, 1982·Toxicology and Applied Pharmacology·R K HindererF J Koschier
Sep 1, 1995·Environmental Health Perspectives·M VillalobosV Pedraza
May 1, 1995·Environmental Health Perspectives·J E Hose, L J Guillette
Apr 1, 1995·Environmental Health Perspectives·S H Safe
May 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·S N BirrellW D Tilley
Oct 1, 1993·Environmental Health Perspectives·T ColbornA M Soto
Jul 1, 1993·Environmental Health Perspectives·A Negro-Vilar
Jul 1, 1993·Environmental Health Perspectives·A Vermeulen
Dec 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·W G SchoonenH J Kloosterboer
Oct 1, 1995·Environmental Health Perspectives·A M SotoF O Serrano
Oct 1, 1995·Environmental Health Perspectives·J P Sumpter, S Jobling
Mar 1, 1996·International Journal of Cancer. Journal International Du Cancer·E KalkhovenB Van Der Burg
Sep 1, 1996·Bulletin of Environmental Contamination and Toxicology·P C Lee, W Lee
Nov 21, 1996·Nature·S GimenoH Komen
Dec 1, 1996·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J R ReelB H Neal
Dec 13, 1996·Science·J Kaiser
Nov 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·C R Nogueira, M M Brentani
Aug 17, 1999·Chemosphere·W G FosterI Chu
Jun 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P GoeganR Vincent

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Citations

Nov 13, 2008·Cytotechnology·Xianghui GongBing Q Shen
May 2, 2000·Food Additives and Contaminants·A M VinggaardJ C Larsen
Jun 16, 2015·Environment International·Ryoiti Kiyama, Yuko Wada-Kiyama
Jun 17, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Marianne ImhofMartin Imhof
Aug 13, 2002·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Thomas Høj Rasmussen, Jesper Bo Nielsen
May 19, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Milan DodigArthur J McCullough
Dec 7, 2007·Human Reproduction Update·P RoyI Huhtaniemi
Oct 19, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mengying ZhangIvonne M C M Rietjens
May 1, 1999·Alternatives to Laboratory Animals : ATLA·I RemezH Veksler

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