Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus ) viscera

PeerJ
Xueqin WangPengcheng Li

Abstract

Mackerel (Pneumatophorus japonicus) viscera contain large amount of protein. We used five proteases to hydrolyze the viscera, and the hydrolysate treated by neutrase exhibited the highest nitrogen recovery (NR). Then we optimized the preparation conditions for mackerel viscera hydrolysate (MVH) by response surface methodology and investigated the antioxidant activity of MVH. The optimal conditions were as follows: enzyme concentration of 1,762.87 U/g, pH of 6.76, temperature of 43.75 °C, extraction time of 6.0 h and water/material ratio of 20.37 (v/w), and the maximum NR was 37.84%. Furthermore, the molecular weight distribution of MVH was almost below 3,000 Da determined by TSK G2000 SWXL gel filtration chromatography, and the MVH exhibited good antioxidant activities in various in vitro assays, including DPPH radical, hydroxyl radical and superoxide anion scavenging activities, reducing power and similar effectivelness as butylated hydroxytoluene and Vitamin E to inhibit lipid peroxidation. The results suggested that MVH could be used as a potential source of antioxidant peptide in food industries.

References

Apr 1, 2000·The Journal of Nutritional Biochemistry·K SuetsunaH Ochi
Aug 14, 2010·Journal of Agricultural and Food Chemistry·Nedra El Hadj AliMoncef Nasri
Nov 10, 2012·Journal of Agricultural and Food Chemistry·Lijun YouMouming Zhao
Apr 22, 2015·Journal of Food Science and Technology·Sadabpong ChoonpicharnHataichanoke Niamsup
Jul 2, 2015·Marine Drugs·Randy Chi Fai CheungJack Ho Wong
Aug 25, 2016·International Journal of Molecular Sciences·Ou YunZhong Han

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Citations

Jun 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xueqin WangPengcheng Li
May 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xueqin WangPengcheng Li
Jun 20, 2019·Protein and Peptide Letters·Sulochana Priya

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Methods Mentioned

BETA
gel filtration

Software Mentioned

Design
Design Expert Trial
Design Expert
SPSS
eqnarray
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