Optimization of bacteriocin-release-protein-induced protein release by Escherichia coli: extracellular production of the periplasmic molecular chaperone FaeE

Applied Microbiology and Biotechnology
F J van der WalJ Luirink

Abstract

Expression of the pCloDF13-encoded bacteriocin-release protein (BRP) results in the release of periplasmic proteins into the culture medium. The BRP-mediated release of a periplasmic protein was investigated and optimized. As a periplasmic model protein, the 50-kDa dimeric E., coli fimbrial molecular chaperone FaeE was used. Plasmids were constructed for the simultaneous expression of the BRP and FaeE, controlled by independently inducible promoters. The efficiency of FaeE release increased when the BRP was targeted by the unstable murein lipoprotein signal peptide, instead of by its own stable signal, peptide. Furthermore, optimal efficacy of FaeE release was found when cells of E. coli strain C600 were used, which harboured one plasmid encoding both FaeE and BRP instead of two separate plasmids and which were cultured at 37 degrees C in broth supplemented with MgCl2. Maximal production levels of 21 mg FaeE/l culture were obtained.

Citations

Jun 25, 2010·Applied Microbiology and Biotechnology·Xiang-Yang Fu
Dec 30, 2009·Journal of Industrial Microbiology & Biotechnology·Niju NarayananC Perry Chou
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Mar 15, 2005·Biotechnology Advances·F J M MergulhãoG A Monteiro
Mar 16, 2005·Protein Expression and Purification·Raja Noor Zaliha Raja Abdul RahmanAbu Bakar Salleh
Feb 21, 2004·FEMS Microbiology Reviews·Mechthild PohlschröderR Wesley Rose
Apr 2, 2013·Research in Microbiology·Roland LloubesStephanie Pommier
Sep 23, 2003·Protein Science : a Publication of the Protein Society·Cornelius BesslerRolf D Schmid

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