Optimization of C16 and C18 fatty alcohol production by an engineered strain of Lipomyces starkeyi

Journal of Industrial Microbiology & Biotechnology
Bonnie A McNeil, David T Stuart

Abstract

The oleaginous yeast Lipomyces starkeyi was engineered for the production of long-chain fatty alcohols by expressing a fatty acyl-CoA reductase, mFAR1, from Mus musculus. The optimal conditions for production of fatty alcohols by this strain were investigated. Increased carbon-to-nitrogen ratios led to efficient C16 and C18 fatty alcohol production from glucose, xylose and glycerol. Batch cultivation resulted in a titer of 1.7 g/L fatty alcohol from glucose which represents a yield of 28 mg of fatty alcohols per gram of glucose. This relatively high level of production with minimal genetic modification indicates that L. starkeyi may be an excellent host for the bioconversion of carbon-rich waste streams, particularly lignocellulosic waste, to C16 and C18 fatty alcohols.

References

Sep 19, 2002·Advances in Applied Microbiology·Colin Ratledge, James P Wynn
Dec 27, 2002·The Journal of General and Applied Microbiology·Hisayuki WatanabeYasuyuki Uzuka
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
Jan 1, 1946·Journal of Bacteriology·R L STARKEY
Nov 3, 2010·FEMS Yeast Research·Hongwei LiuHanfa Zou
Mar 8, 2011·Journal of Biotechnology·Jintao LinZongbao K Zhao
Jan 5, 2012·Microbial Cell Factories·Giusy Manuela AdamoMarina Lotti
Apr 25, 2013·Current Opinion in Biotechnology·Jens NielsenJack Pronk
Jun 25, 2013·Applied Microbiology and Biotechnology·Aiqiu LiuXuefeng Lu
Feb 22, 2014·Bioresource Technology·Andréia AnschauTelma T Franco
Apr 15, 2014·Current Genetics·Christopher H CalveyThomas W Jeffries
Jul 16, 2014·Biotechnology for Biofuels·Lun YaoXuefeng Lu
Aug 15, 2014·Biotechnology and Bioengineering·Xiaoling Tang, Wei Ning Chen
Dec 4, 2014·Metabolic Engineering·Xueyang FengHuimin Zhao
Sep 1, 2015·Journal of Industrial Microbiology & Biotechnology·Sandy FilletJosé L Adrio
Nov 19, 2015·Bioresource Technology·Christopher H CalveyThomas W Jeffries
May 24, 2016·Biotechnology for Biofuels·Guokun WangShulin Chen
May 26, 2016·Nature Communications·Yongjin J ZhouJens Nielsen
Oct 30, 2016·Metabolic Engineering·Rodrigo Ledesma-AmaroJean-Marc Nicaud
Jul 28, 2016·PloS One·Varsha VijaySharon J Smith
Jul 29, 2016·Microbial Cell Factories·Yilan LiuYanhe Ma
Nov 2, 2016·Biotechnology for Biofuels·Wei WangMin Zhang
May 13, 2017·Biotechnology and Bioengineering·José L Adrio

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Citations

Sep 14, 2018·World Journal of Microbiology & Biotechnology·Bonnie A McNeil, David T Stuart
Jun 18, 2020·Metabolic Engineering Communications·Lauren T CordovaHal S Alper
Dec 15, 2020·FEMS Microbiology Reviews·Justyna Ruchala, Andriy A Sibirny
Dec 22, 2020·Frontiers in Bioengineering and Biotechnology·Anagha KrishnanDavid T Stuart
Feb 13, 2021·Frontiers in Bioengineering and Biotechnology·Yilan LiuRadhakrishnan Mahadevan
Nov 28, 2020·Current Opinion in Biotechnology·Jae Won LeeYong-Su Jin
Sep 25, 2018·Current Opinion in Biotechnology·Holly EkasHal S Alper
Feb 26, 2021·Biotechnology Advances·Atrayee ChattopadhyayMrinal K Maiti
Mar 16, 2019·Current Opinion in Biotechnology·Michael SpagnuoloMark Blenner

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Methods Mentioned

BETA
PCR
genetic modifications
genetic modification

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