Optimization of carboxymethyl chitosan synthesis using response surface methodology and desirability function

International Journal of Biological Macromolecules
Andrea de Lacerda BukzemDiego Palmiro R Ascheri

Abstract

In this paper, chitosan was reacted with monochloroacetic acid under alkaline conditions to prepare carboxymethyl chitosan. A 2(3) full-factorial central composite design was applied to evaluate the effect of molar ratio sodium hydroxide (NaOH)/Chitosan (Ch), time and molar ratio monochloroacetic acid (MCA)/Chitosan (Ch) on the reaction yield and on the characteristics of carboxymethyl chitosan such as average degree of substitution (DS¯) and solubility. An optimization strategy based on response surface methodology was used together with the desirability function approach to optimize this process. The occurrence of carboxymethylation was evidenced by FTIR and (1)H NMR spectroscopy. The optimum conditions for carboxymethylation process were found to be 12.4, 10.6h and 5 for molar ratio sodium hydroxide (NaOH)/Chitosan (Ch), time and molar ratio monochloroacetic acid (MCA)/Chitosan (Ch), respectively. Under these optimal conditions, it was possible to obtain carboxymethyl chitosan with DS¯ of 1.86 and solubility of 99.6%. X-ray diffraction and thermogravimetry analysis showed that crystallinity and thermal stability of derivatives was lower than chitosan and decreased with increase of DS¯.

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Citations

Jun 11, 2016·International Journal of Biological Macromolecules·Rania A HashadSamar Mansour
Jan 4, 2019·Tissue Engineering and Regenerative Medicine·Shailendra Singh SheraRathindra Mohan Banik
Jan 17, 2020·International Journal of Molecular Sciences·Wenqian WangKai Zhao
Nov 1, 2020·International Journal of Biological Macromolecules·Andrea de Lacerda BukzemSérgio Paulo Campana-Filho
Feb 14, 2021·International Journal of Biological Macromolecules·Alireza ForoughniaNadiya Koukabi
Mar 7, 2021·International Journal of Pharmaceutics·Sante Di GioiaMassimo Conese
Jul 14, 2020·International Journal of Biological Macromolecules·Ahmed R SofyMahmoud R Sofy
Apr 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Farideh DavaniKamiar Zomorodian
Apr 30, 2021·Biomacromolecules·Juho Antti SirviöSvitlana Filonenko
Jul 31, 2021·Journal of Biomaterials Science. Polymer Edition·Samuel Ogbeide Ebhodaghe
Aug 21, 2019·Carbohydrate Polymers·Luã Caldas de OliveiraLúcia de Fátima Henriques Lourenço

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