Optimization of cell morphology measurement via single-molecule tracking PALM.

PloS One
Nicholas A FrostThomas A Blanpied

Abstract

In neurons, the shape of dendritic spines relates to synapse function, which is rapidly altered during experience-dependent neural plasticity. The small size of spines makes detailed measurement of their morphology in living cells best suited to super-resolution imaging techniques. The distribution of molecular positions mapped via live-cell Photoactivated Localization Microscopy (PALM) is a powerful approach, but molecular motion complicates this analysis and can degrade overall resolution of the morphological reconstruction. Nevertheless, the motion is of additional interest because tracking single molecules provides diffusion coefficients, bound fraction, and other key functional parameters. We used Monte Carlo simulations to examine features of single-molecule tracking of practical utility for the simultaneous determination of cell morphology. We find that the accuracy of determining both distance and angle of motion depend heavily on the precision with which molecules are localized. Strikingly, diffusion within a bounded region resulted in an inward bias of localizations away from the edges, inaccurately reflecting the region structure. This inward bias additionally resulted in a counterintuitive reduction of measured diff...Continue Reading

References

Aug 1, 1994·Journal of Neuroscience Research·J Takács, J Hámori
Jan 1, 1994·The Journal of Neuropsychiatry and Clinical Neurosciences·D R WeinbergerK F Berman
Aug 1, 1995·Biophysical Journal·M J Saxton
Feb 5, 2002·Annual Review of Physiology·Esther A NimchinskyKarel Svoboda
Apr 20, 2002·Biophysical Journal·Russell E ThompsonWatt W Webb
Apr 3, 2003·Nature Reviews. Neuroscience·Daniel Choquet, Antoine Triller
Jan 30, 2004·Biophysical Journal·Raimund J OberE Sally Ward
Jun 11, 2004·Biophysical Journal·Brian L SpragueJames G McNally
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sripad RamRaimund J Ober
Mar 22, 2006·Nature Biotechnology·Nadya G GurskayaKonstantin A Lukyanov
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Bianxiao CuiSteven Chu
Oct 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·Samuel T HessJoshua Zimmerberg
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hari ShroffEric Betzig
Jan 15, 2008·Nature Methods·Suliana ManleyJennifer Lippincott-Schwartz
Feb 21, 2008·Annual Review of Neuroscience·Jennifer N Bourne, Kristen M Harris
Jul 8, 2008·Nature Methods·Arnauld SergéDidier Marguet
Nov 13, 2008·Nature Reviews. Molecular Cell Biology·Marta Fernández-Suárez, Alice Y Ting
Dec 17, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Asa GrunditzThomas G Oertner
Jan 1, 2009·Nature Methods·Jennifer Lippincott-Schwartz, Suliana Manley
Jan 27, 2009·Nature Methods·Sean A McKinneyLoren L Looger
Feb 14, 2009·Nature Protocols·Travis J GouldSamuel T Hess
Mar 31, 2009·Trends in Cell Biology·Thomas M Newpher, Michael D Ehlers
Jul 30, 2010·Current Opinion in Neurobiology·Nicholas A FrostThomas A Blanpied
Aug 18, 2010·Biophysical Journal·Gregory GiannoneLaurent Cognet
Oct 29, 2010·Nature Communications·Takashi UemuraSusumu Kitagawa
Feb 25, 2011·Nature Neuroscience·Peter PenzesKevin M Woolfrey
May 10, 2011·Nature Methods·Sara A JonesXiaowei Zhuang
Jun 28, 2011·Cell·Anne J Ridley
Sep 6, 2011·Biophysical Journal·Nicolai T UrbanU Valentin Nägerl
Oct 8, 2011·PloS One·Marianne RennerAntoine Triller

❮ Previous
Next ❯

Citations

Apr 18, 2014·Nature Reviews. Cancer·James R W ConwayPaul Timpson
Feb 14, 2014·Histochemistry and Cell Biology·Virgile Adam
Jun 15, 2014·FEBS Letters·A ShivanandanA Radenovic
Nov 9, 2015·The European Journal of Neuroscience·Harold D MacGillavryThomas A Blanpied
Mar 10, 2015·Experimental Cell Research·Harold D MacGillavry, Casper C Hoogenraad
Dec 30, 2014·Frontiers in Neuroanatomy·Max AdrianLukas C Kapitein
Nov 22, 2014·Frontiers in Synaptic Neuroscience·Francisco J Barrantes
Oct 5, 2016·The Journal of Cell Biology·David AlbrechtHelge Ewers
Feb 28, 2015·Cell and Tissue Research·Wim VandenbergPeter Dedecker
Jun 5, 2014·Journal of Cell Science·José I ValenzuelaAndrés Couve
Nov 28, 2014·Current Protocols in Neuroscience·Harold D MacGillavry, Thomas A Blanpied
May 8, 2020·PloS One·Julie A CassJulie Theriot
Nov 6, 2018·The Journal of Physical Chemistry. B·Xihong XieTai-Yen Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
electron microscopy
super-resolution

Software Mentioned

Matlab
ImageJ
Origin

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.