Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic ethanol production from corn stover

Biotechnology for Biofuels
Yong-Hao LiXin-Qing Zhao

Abstract

Cellulolytic enzymes produced by Trichoderma reesei are widely studied for biomass bioconversion, and enzymatic components vary depending on different inducers. In our previous studies, a mixture of glucose and disaccharide (MGD) was developed and used to induce cellulase production. However, the enzymatic profile induced by MGD is still not defined, and further optimization of the enzyme cocktail is also required for efficient ethanol production from lignocellulosic biomass. In this study, cellulolytic enzymes produced by T. reesei Rut C30 using MGD and alkali-pretreated corn stover (APCS) as inducer were compared. Cellular secretome in response to each inducer was analyzed, which revealed a similar enzyme profile. However, significant difference in the content of cellulases and xylanase was detected. Although MGD induction enhanced β-glucosidase production, its activity was still not sufficient for biomass hydrolysis. To overcome such a disadvantage, aabgl1 encoding β-glucosidase in Aspergillus aculeatus was heterologously expressed in T. reesei Rut C30 under the control of the pdc1 promoter. The recombinant T. reesei PB-3 strain showed an improved β-glucosidase activity of 310 CBU/mL in the fed-batch fermentation, 71-folds h...Continue Reading

References

Oct 26, 2002·BMC Biotechnology·Giovanna MasonGian Paolo Accotto
Oct 4, 2008·Nature Protocols·Caroline B MichielseArthur F J Ram
Aug 14, 2010·Science·Chris SomervilleStephen P Long
Oct 15, 2011·Microbiology·Robyn Peterson, Helena Nevalainen
Oct 17, 2012·Bioresource Technology·Reeta Rani SinghaniaAshok Pandey
Aug 19, 2014·Applied Microbiology and Biotechnology·In Jung KimKyoung Heon Kim
Nov 3, 2015·Biotechnology and Bioengineering·Linda LehmannTimothy Hobley
Dec 3, 2016·Nucleic Acids Research·Amanda Reider ApelAindrila Mukhopadhyay
Mar 8, 2017·Biotechnology for Biofuels·Constantinos KatsimpourasEvangelos Topakas
May 31, 2017·Microbial Biotechnology·Irina S Druzhinina, Christian P Kubicek

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Datasets Mentioned

BETA
D64088.1
Z32698.1
AF139061.1

Methods Mentioned

BETA
PCR

Software Mentioned

Maxquant
SignalP

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