Abstract
Statistical experimental designs were employed to optimize culture conditions for cold-adapted lysozyme production of a psychrophilic yeast Debaryomyces hansenii. In the first step of optimization using Plackett-Burman design (PBD), peptone, glucose, temperature, and NaCl were identified as significant variables that affected lysozyme production, the formula was further optimized using a four factor central composite design (CCD) to understand their interaction and to determine their optimal levels. A quadratic model was developed and validated. Compared to the initial level (18.8 U/mL), the maximum lysozyme production (65.8 U/mL) observed was approximately increased by 3.5-fold under the optimized conditions. Cold-adapted lysozymes production was first optimized using statistical experimental methods. A 3.5-fold enhancement of microbial lysozyme was gained after optimization. Such an improved production will facilitate the application of microbial lysozyme. Thus, D. hansenii lysozyme may be a good and new resource for the industrial production of cold-adapted lysozymes.
References
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M KikuchiM Ikehara
Sep 1, 1984·Molecular and Cellular Biochemistry·P Jollès, J Jollès
Feb 1, 1994·Preparative Biochemistry·B Myrnes, A Johansen
Nov 5, 1997·Applied and Environmental Microbiology·C PristaJ Ramos
Dec 24, 1997·Comparative Biochemistry and Physiology. Part A, Physiology·N J Russell
Jun 1, 2000·Applied and Environmental Microbiology·D S NicholsT A McMeekin
Jun 28, 2001·The Journal of Biological Chemistry·A RauR Hilgenfeld
Jul 25, 2003·International Journal of Food Microbiology·Frances M Nattress, Lynda P Baker
Mar 24, 2004·Nature Reviews. Microbiology·Georges Feller, Charles Gerday
Jul 15, 2004·Extremophiles : Life Under Extreme Conditions·Tatiana GroudievaGarabed Antranikian
Sep 15, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Qing-Gang XueJerome F La Peyre
Jun 30, 2006·Bioresource Technology·Pardeep Kumar, T Satyanarayana
May 15, 2007·Bioresource Technology·Quanfu WangGuangyou Li
Dec 6, 2008·Extremophiles : Life Under Extreme Conditions·Rosa Margesin
Jun 15, 2010·Bioresource Technology·Shu LiuLi Chen
Nov 12, 2010·Bioresource Technology·Gyan PrakashMala Rao
Mar 3, 2011·Marine Biotechnology·Jianguo LiuZhanfeng Cui