OPTIMIZATION OF COLLAGEN-ELASTIN-LIKE POLYPEPTIDE-BIOGLASS SCAFFOLD COMPOSITION FOR OSTEOGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS.

Materialia
Bhuvaneswari GurumurthyAmol V Janorkar

Abstract

We have developed a multicomponent hydrogel scaffold that can mimic the bone extracellular matrix by incorporating collagen, elastin-like polypeptide (ELP), and Bioglass. We examined the effects of Bioglass addition to collagen-ELP scaffolds on mechanical properties, physical characteristics, and in vitro osteogenic differentiation, by varying the Bioglass amount and particle size. Response surface methodology with a central composite design predicted 5 mg (6.6 mg/mL) Bioglass with a particle size of 142 ± 5 μm as the optimal amount and particle size to be mixed with 6 mg/mL collagen and 18 mg/mL ELP to obtain a combination of maximized compressive properties. Swelling ratio and FTIR spectroscopy indicated lower hydrophilicity and the presence of hydrophobic and secondary interactions between collagen, ELP, and Bioglass. Scanning electron microscopy showed a nanofibrous morphology of intermingled collagen-ELP-Bioglass network. In vitro osteogenic characterization using human adipose-derived stem cells revealed increased cell attachment and proliferation with increased ALP activity, osteocalcin content, and mineralized deposit formation during a three-week culture. Numerous mineralized deposits composed of calcium and phosphorou...Continue Reading

References

Aug 11, 1998·Journal of Biomedical Materials Research·L L Hench
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Aug 21, 2007·Macromolecular Bioscience·Aparna R SarasamSundararajan V Madihally
Feb 26, 2008·Acta Biomaterialia·Biraja P KanungoLorna J Gibson
Sep 23, 2008·Injury·Peter V GiannoudisDavid Marsh
Feb 4, 2010·Tissue Engineering. Part B, Reviews·Nasim AnnabiFariba Dehghani
May 18, 2012·Dental Materials : Official Publication of the Academy of Dental Materials·Marco C BottinoGregg M Janowski
Jan 24, 2013·Critical Reviews in Biomedical Engineering·Ami R AminiSyam P Nukavarapu
Feb 22, 2013·Journal of Biomedical Materials Research. Part a·Shruti S AmruthwarAmol V Janorkar
May 31, 2013·Journal of Biomedical Materials Research. Part a·Daniela PasquiRolando Barbucci
Mar 4, 2014·International Journal of Biological Macromolecules·Atefeh BroumandSasan Mirzakhanlouei
Jan 1, 2013·Global Cardiology Science & Practice·Ibrahim M El-Sherbiny, Magdi H Yacoub
Sep 13, 2014·Journal of the Mechanical Behavior of Biomedical Materials·Wei LiAldo R Boccaccini
Nov 29, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Teng LongZhen-An Zhu
Aug 11, 2015·International Journal of Biological Macromolecules·Akbar Esmaeili, Azadeh Asgari
Sep 1, 2015·Biomaterials Science·K Jahan, M Tabrizian
Sep 12, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Rubaiya Yunus BashaMukesh Doble
Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Esmaeel SharifiJafar Ai
Jan 12, 2016·Journal of Biomedical Materials Research. Part a·Gengbo LiuVipuil Kishore
Apr 30, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Chhavi SharmaNarayan Chandra Mishra
Aug 16, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Bhuvaneswari GurumurthyAmol V Janorkar
Apr 19, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Payam RadaeiSaeid Vakilian
May 18, 2018·Journal of the Mechanical Behavior of Biomedical Materials·Bhuvaneswari GurumurthyAmol V Janorkar

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