Optimization of culture conditions and bench-scale production of L-asparaginase by submerged fermentation of Aspergillus terreus MTCC 1782

Journal of Microbiology and Biotechnology
Baskar Gurunathan, Renganathan Sahadevan

Abstract

Optimization of culture conditions for L-asparaginase production by submerged fermentation of Aspergillus terreus MTCC 1782 was studied using a 3-level central composite design of response surface methodology and artificial neural network linked genetic algorithm. The artificial neural network linked genetic algorithm was found to be more efficient than response surface methodology. The experimental L-asparaginase activity of 43.29 IU/ml was obtained at the optimum culture conditions of temperature 35 degrees C, initial pH 6.3, inoculum size 1% (v/v), agitation rate 140 rpm, and incubation time 58.5 h of the artificial neural network linked genetic algorithm, which was close to the predicted activity of 44.38 IU/ml. Characteristics of L-asparaginase production by A. terreus MTCC 1782 were studied in a 3 L bench-scale bioreactor.

Citations

Oct 12, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Thangavelu MuthukumarThotapalli Parvathaleswara Sastry
Dec 24, 2015·Critical Reviews in Biotechnology·André Moreni LopesAdalberto Pessoa
Jan 16, 2015·Journal of Materials Science. Materials in Medicine·G BaskarT Muthukumar
Nov 22, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Jorge Javier Muso CachumbaSilvio Silvério Da Silva
Oct 16, 2018·Recent Patents on Biotechnology·Tania PaulTarun Kanti Bandyopadhyay
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May 20, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Samuel L CardosoPérola O Magalhães

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